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. 1979 Aug;175(1):111-2.
doi: 10.1007/BF00267862.

Low ribonuclease activity in cellular lysates of osmotic sensitive Saccharomyces cerevisiae mutants

P V Venkov

Low ribonuclease activity in cellular lysates of osmotic sensitive Saccharomyces cerevisiae mutants

P V Venkov. Mol Gen Genet. 1979 Aug.

Abstract

Cellular lysates with very low total ribonuclease activities are obtained by lysis of Saccharomyces cerevisiae VY1160 osmotic sensitive mutant cells in 1% sorbitol solution. These lysates could be used for isolation of intact polysomes and messenger RNA molecules, or for studying of specific ribonucleases.

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