Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Jul 19;24(1):711.
doi: 10.1186/s12879-024-09608-2.

Some virulence genes are associated with antibiotic susceptibility in Enterobacter cloacae complex

Fatemeh Mosaffa  1 , Fereshteh Saffari  1 , Mahin Veisi  1 , Omid Tadjrobehkar  2
Affiliations

Some virulence genes are associated with antibiotic susceptibility in Enterobacter cloacae complex

Fatemeh Mosaffa et al. BMC Infect Dis. .

Erratum in

Abstract

Background: Enterobacter cloacae complex (ECC) including different species are isolated from different human clinical samples. ECC is armed by many different virulence genes (VGs) and they were also classified among ESKAPE group by WHO recently. The present study was designed to find probable association between VGs and antibiotic susceptibility in different ECC species.

Methods: Forty-five Enterobacter isolates that were harvested from different clinical samples were classified in four different species. Seven VGs were screened by PCR technique and antibiotic susceptibility assessment was performed by disk-diffusion assay.

Result: Four Enterobacter species; Enterobacter cloacae (33.3%), Enterobacter hormaechei (55.6%), Enterobacter kobei (6.7%) and Enterobacter roggenkampii (4.4%) were detected. Minimum antibiotic resistance was against carbapenem agents and amikacin even in MDR isolates. 33.3% and 13.3% of isolates were MDR and XDR respectively. The rpoS (97.8%) and csgD (11.1%) showed maximum and minimum frequency respectively. Blood sample isolated were highly virulent but less resistant in comparison to the other sample isolates. The csgA, csgD and iutA genes were associated with cefepime sensitivity.

Conclusion: The fepA showed a predictory role for differentiating of E. hormaechei from other species. More evolved iron acquisition system in E. hormaechei was hypothesized. The fepA gene introduced as a suitable target for designing novel anti-virulence/antibiotic agents against E. hormaechei. Complementary studies on other VGs and ARGs and with bigger study population is recommended.

Keywords: Enterobacter cloacae complex; Antibiotic resistance; Antivirulence; MDR; Virulence gene.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Frequency of resistance against different antibiotic agents in Enterobacter isolates. The trimethoprim/sulfamethoxazole combination referred as Cotrimoxazole
Fig. 2
Fig. 2
Frequency of csgA, csgD and iutA genes among antibiotic resistance isolates in comparison to the antibiotic sensitive isolates. The trimethoprim/sulfamethoxazole combination referred as Cotrimoxazole. The p-value was derived from Fischer’s exact test analysis. *p ≤ 0.05, **p ≤ 0.06 and ***p ≤ 0.09
See this image and copyright information in PMC

References

    1. Tindall, BJ, G Sutton, and GM Garrity, Enterobacter aerogenes Hormaeche and Edwards 1960 (Approved Lists 1980) and Klebsiella mobilis Bascomb et al. 1971 (Approved Lists 1980) share the same nomenclatural type (ATCC 13048) on the Approved Lists and are homotypic synonyms, with consequences for the name Klebsiella mobilis Bascomb et al. 1971 (Approved Lists 1980). Int J Syst Evol Microbiol, 2017. 67(2):502–504.10.1099/ijsem.0.001572. - PubMed
    1. Wu, W., Y. Feng, and Z. Zong, Precise species identification for enterobacter: A genome sequence-based study with reporting of two novel species, enterobacter quasiroggenkampii sp. nov. and Enterobacter quasimori sp. nov. mSystems, 2020. 5(4): 10.1128/msystems.00527–20.10.1128/mSystems.00527-20. - PMC - PubMed
    1. Davin-Regli A, Lavigne J-P, Pages J-M. Enterobacter spp.: Update on taxonomy, clinical aspects, and emerging antimicrobial resistance. Clin Microbiol Rev. 2019;32(4):e00002-19. 10.1128/CMR.00002-19. - PMC - PubMed
    1. Mancuso G, et al. Bacterial antibiotic resistance: The most critical pathogens. Pathogens. 2021;10(10):1310. 10.3390/pathogens10101310. - PMC - PubMed
    1. Davin-Regli A, Pages JM. Enterobacter aerogenes and Enterobacter cloacae; versatile bacterial pathogens confronting antibiotic treatment. Front Microbiol. 2015;6:392. 10.3389/fmicb.2015.00392. - PMC - PubMed

MeSH terms

LinkOut - more resources