Complete genome sequence of Microbacterium foliorum phage Curie, a podovirus isolated from soil in Spokane, Washington

Abstract

Bacteriophage Curie is a podovirus that infects Microbacterium foliorum. The Curie genome spans 16,810 bp, has 90 bp terminal inverted repeats, and includes 23 protein-coding genes. Its genome architecture resembles phage PineapplePizza and other phi29-like phages. Together, Curie and PineapplePizza form a new actinobacteriophage Cluster GI.

Keywords: bacteriophages.

Fig 1

Phage Curie virion and genome structure. (A) Transmission electron micrograph of Curie, negatively stained with 1% uranyl acetate. (B) Curie genome compared to PineapplePizza and Anjali, a representative of Cluster FD. Gray bands between genomes show tBLASTx (v.2.15) alignments (7). The shade of each band indicates percent identity according to the grayscale bar, 24%–100%. Genes (arrows and arrowheads) are filled with the same color if they were members of gene families formed by Phamerator (8), using sequences in the Actino_Draft database version 552 (9). Genes filled with gray encode proteins with at least one predicted transmembrane domain. Endolysins are labeled with the domain they contain: M15 peptidase, M23 peptidase, or N-acetylmuramoyl-L-alanine amidase. The genome alignment was generated with EasyFig v.2.2, filtering for tBLASTx e-values smaller than 10⁻⁵⁰ (10).