Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1985 Oct;52(4):505-13.
doi: 10.1038/bjc.1985.221.

Cloning of human lung cancer cells

G A Walls, P R Twentyman
Free PMC article
Comparative Study

Cloning of human lung cancer cells

G A Walls et al. Br J Cancer. 1985 Oct.
Free PMC article

Abstract

We have carried out a comparison of two different methods for cloning human lung cancer cells. The method of Courtenay & Mills (1978) generally gave higher plating efficiencies (PE) than the method of Carney et al. (1980). The number of colonies increased with incubation time in both methods and the weekly medium replenishment in the Courtenay method was advantageous for longer incubation times of several weeks. In the Courtenay method, the use of August rat red blood cells (RBC) and low oxygen tension were both found to be necessary factors for maximum plating efficiency. The usefulness of heavily irradiated feeder cells in improving PE is less certain; each cell type may have its own requirement.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Aust J Exp Biol Med Sci. 1966 Jun;44(3):287-99 - PubMed
    1. Blood. 1971 Sep;38(3):353-9 - PubMed
    1. J Natl Cancer Inst. 1972 Dec;49(6):1705-12 - PubMed
    1. Br J Cancer. 1976 Jul;34(1):39-45 - PubMed
    1. Science. 1977 Jul 29;197(4302):461-3 - PubMed

Publication types