[Comparison of different methods for measuring nucleic acids and protein in the biomass, half-products and preparations of E. coli and yeast]

Abstract

In the E. coli biomass, half-products and RNA preparations a comparative measurement of nucleic acids and protein was carried out using different methods. As a result, the following methods can be recommended: to assay RNA in the biomass--spectrophotometry, to determine RNA in half-products and preparations--the phosphate method, to assess DNA--the diphenyl amine method, and to measure protein--according to Lowry. The same methods can be recommended to estimate RNA from baker's yeast.