Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2024 Jul 13;13(14):1190.
doi: 10.3390/cells13141190.

Advancements in Research on Genetic Kidney Diseases Using Human-Induced Pluripotent Stem Cell-Derived Kidney Organoids

Do Hyun Na  1   2 , Sheng Cui  1 , Xianying Fang  1 , Hanbi Lee  1   2 , Sang Hun Eum  1   3 , Yoo Jin Shin  1 , Sun Woo Lim  1 , Chul Woo Yang  1   2 , Byung Ha Chung  1   2
Affiliations
Review

Advancements in Research on Genetic Kidney Diseases Using Human-Induced Pluripotent Stem Cell-Derived Kidney Organoids

Do Hyun Na et al. Cells. .

Abstract

Genetic or hereditary kidney disease stands as a pivotal cause of chronic kidney disease (CKD). The proliferation and widespread utilization of DNA testing in clinical settings have notably eased the diagnosis of genetic kidney diseases, which were once elusive but are now increasingly identified in cases previously deemed CKD of unknown etiology. However, despite these diagnostic strides, research into disease pathogenesis and novel drug development faces significant hurdles, chiefly due to the dearth of appropriate animal models and the challenges posed by limited patient cohorts in clinical studies. Conversely, the advent and utilization of human-induced pluripotent stem cells (hiPSCs) offer a promising avenue for genetic kidney disease research. Particularly, the development of hiPSC-derived kidney organoid systems presents a novel platform for investigating various forms of genetic kidney diseases. Moreover, the integration of the CRISPR/Cas9 technique into this system holds immense potential for efficient research on genetic kidney diseases. This review aims to explore the applications of in vitro kidney organoids generated from hiPSCs in the study of diverse genetic kidney diseases. Additionally, it will delve into the limitations of this research platform and outline future perspectives for advancing research in this crucial area.

Keywords: gene editing; genetic kidney disease; human-induced pluripotent stem cell (hiPSC); kidney organoid.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Scheme of process for the modeling of genetic kidney disease using hiPSC. * hiPSC; human-induced pluripotent stem cell.
See this image and copyright information in PMC

References

    1. Jha V., Garcia-Garcia G., Iseki K., Li Z., Naicker S., Plattner B., Saran R., Wang A.Y.-M., Yang C.-W. Chronic kidney disease: Global dimension and perspectives. Lancet. 2013;382:260–272. doi: 10.1016/S0140-6736(13)60687-X. - DOI - PubMed
    1. Kovesdy C.P. Epidemiology of chronic kidney disease: An update 2022. Kidney Int. Suppl. 2022;12:7–11. doi: 10.1016/j.kisu.2021.11.003. - DOI - PMC - PubMed
    1. Devuyst O., Knoers N.V., Remuzzi G., Schaefer F. Rare inherited kidney diseases: Challenges, opportunities, and perspectives. Lancet. 2014;383:1844–1859. doi: 10.1016/S0140-6736(14)60659-0. - DOI - PMC - PubMed
    1. Participants K.C. Genetics in chronic kidney disease: Conclusions from a Kidney Disease: Improving Global Outcomes (KDIGO) Controversies Conference. Kidney Int. 2022;101:1126–1141. doi: 10.1016/j.kint.2022.03.019. - DOI - PMC - PubMed
    1. Hong Y.A., Ban T.H., Kang C.-Y., Hwang S.D., Choi S.R., Lee H., Jung H.-Y., Kim K., Kwon Y.E., Kim S.H., et al. Trends in epidemiologic characteristics of end-stage renal disease from 2019 Korean Renal Data System (KORDS) Kidney Res. Clin. Pract. 2021;40:52–61. doi: 10.23876/j.krcp.20.202. - DOI - PMC - PubMed

Publication types

LinkOut - more resources