Correction: Alves et al. WNK2 Inhibits Autophagic Flux in Human Glioblastoma Cell Line. Cells 2020, 9, 485

Abstract

Error in Figure [...].

Figure 2

Evaluation of proteins involved in the mammalian target of rapamycin (mTOR) pathway by western blot. A172 WT, A172 EV, and A172 WNK2 cell lines were treated with bafilomycin A1 (BAF, 20 nM), starvation (EBSS medium), or everolimus (EVERO, 10 nM) for 4 (A) and 6 h (B). The protein extract was evaluated for phosphorylation of mTOR and its substrates p-p70S6K and p-4EBP1 by western blot. Normalized densitometric band intensities of mTOR activity used α-tubulin as an endogenous loading control. For the substrates p-p70S6K and p-4EBP1, β-actin was used as an endogenous control. The graphs are representative of two independent biological experiments. n.s.: Not significant.