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. 2024 Jul 12;46(7):7339-7352.
doi: 10.3390/cimb46070435.

Analysis of Intestinal Bacterial Microbiota in Individuals with and without Chronic Low Back Pain

Antonio Martins Tieppo  1 , Júlia Silva Tieppo  2 , Luiz Antonio Rivetti  3
Affiliations

Analysis of Intestinal Bacterial Microbiota in Individuals with and without Chronic Low Back Pain

Antonio Martins Tieppo et al. Curr Issues Mol Biol. .

Abstract

Low back pain is a health problem that represents the greatest cause of years lived with disability. This research seeks to evaluate the bacterial composition of the intestinal microbiota of two similar groups: one with chronic low back pain (PG) and the control group (CG). Clinical data from 73 participants and bacterial genome sequencing data from stool samples were analyzed. There were 40 individuals in PG and 33 in CG, aged between 20 and 50 years and with a body mass index of up to 30 kg/m2. Thus, the intragroup alpha diversity and intergroup beta diversity were analyzed. The significant results (p < 0.05) showed greater species richness in PG compared to CG. Additionally, a greater abundance of the species Clostridium difficile in PG was found along with 52 species with significantly different average relative abundances between groups (adjusted p < 0.05), with 36 more abundant species in PG and 16 in CG. We are the first to unveil significant differences in the composition of the intestinal bacterial microbiota of individuals with chronic low back pain who are non-elderly, non-obese and without any other serious chronic diseases. It could be a reference for a possible intestinal bacterial microbiota signature in chronic low back pain.

Keywords: chronic low back pain; inflammation; intestinal microbiome; intestinal microbiota.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
The ar-Ab of bacterial species of the phylum Firmicutes between CG and PG.
Figure 2
Figure 2
The ar-Ab of bacterial species of the phylum Bacteroidetes between CG and PG.
Figure 3
Figure 3
The ar-Ab of the bacterial species of the phylum Proteobacteria between CG and PG.
Figure 4
Figure 4
The ar-Ab of the bacterial species of the phylum Actinobacteria between CG and PG.
Figure 5
Figure 5
The ar-Ab of bacterial species from other phyla between CG and PG.
Figure 6
Figure 6
Circular compositional bar chart sorted using the Bray–Curtis sorting angle [35]. This graphic representation of the circle of outer lines shows each participant, with the green rectangles representing CG and the red ones representing PG. In the central projection, the pattern found in the survey is represented according to the color palette, showing 15 more abundant species (6 in CG, 9 in PG) out of the 52 revealed through the diversity indices applied with adjusted p differences < 0.05.
See this image and copyright information in PMC

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