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. 2024 Jun 22;10(7):442.
doi: 10.3390/jof10070442.

First Data on the Investigation of Gut Yeasts in Hermit Beetle (Osmoderma barnabita Motschulsky, 1845) Larvae in Lithuania

Jurgita Švedienė  1 , Vita Raudonienė  1 , Goda Mizerienė  2 , Jolanta Rimšaitė  3 , Sigitas Algis Davenis  3 , Povilas Ivinskis  3
Affiliations

First Data on the Investigation of Gut Yeasts in Hermit Beetle (Osmoderma barnabita Motschulsky, 1845) Larvae in Lithuania

Jurgita Švedienė et al. J Fungi (Basel). .

Abstract

In this study, yeasts from the gut of O. barnabita larvae were isolated and molecularly identified. It is worth noting that this research provides the first analysis of the gut yeast community in O. barnabita larvae in Lithuania, which is a significant contribution to the field. Two hermit-like L3-praepupa instars were collected from a decaying oak log in Lithuania. The isolation, morphology, biochemistry, and physiology of the yeast isolates were characterized using standards commonly employed in yeast taxonomy studies. The isolates were identified by sequencing the large subunit (26S) rDNA (D1/D2 domain of the LSU). All gut compartments were colonized by the yeast. A total of 45 yeast strains were obtained from the gut of both O. barnabita larvae, with 23 strains originating from Larva 1, 16 strains from Larva 2, and 6 strains from the galleries. According to our identification results of the 45 yeast strains, most of the species were related to Ascomycota, with most of them belonging to the Saccharomycetales order. Yeasts of the genera Candida, Debaryomyces, Meyerozyma, Priceomyces, Schwanniomyces, Spencermartinsiella, Trichomonascus, and Blastobotrys were present in gut of O. barnabita larvae. Species of the Trichosporonales order represented the Basidiomycota phylum.

Keywords: beetle; diversity; gut; identification; larvae; yeasts.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Osmoderma barnabita Larva 1 on rotten wood (photo S. A. Davenis).
Figure 2
Figure 2
Morphological diversity of yeast from the gut of O. barnabita Larva 1: (A) foregut, (B) midgut, and (C) hindgut on YM agar.
Figure 3
Figure 3
The ability of yeast to assimilate some carbon sources (in percentages).
Figure 4
Figure 4
Heatmap of yeasts isolated from the gut of Osmoderma barnabita and the galleries.
Figure 5
Figure 5
Percentage of yeast species isolated from each larva.
Figure 6
Figure 6
Phylogenetic relationships among ascomycetous yeast genera.
Figure 7
Figure 7
Phylogenetic relationships among basidiomycetous yeast genera.
See this image and copyright information in PMC

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