Dopamine decreases release of luteinizing hormone releasing hormone from superfused rat mediobasal hypothalamus

Abstract

The effects of dopamine hydrochloride (DA) on the releases of LHRH and LH were examined in a serial sequential double chamber perifusion system by perifusing the mediobasal hypothalamus including the preoptic area and/or pituitaries excised from diestrus female rats. DA, perifused at a dose of 4.2 X 10(-4) M, significantly (p less than 0.05) lowered LH secretion from the pituitary in series with the hypothalamus 40-80% below the preinfusion level, but it had no effect on LH efflux from the pituitary perifused alone. DA also significantly (p less than 0.05) reduced the LHRH level 20-40% below the initial level. Perifusion with the DA receptor blocker haloperidol at a concentration of 10(-6) M abolished the suppressive effect of DA on LH secretion. These findings indicate that DA suppressed hypothalamic LHRH release, resulting in decrease in LH secretion from the pituitary in diestrus rats.