Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Jul 4;13(7):622.
doi: 10.3390/antibiotics13070622.

Antiparasitic Activity of Isolated Fractions from Parthenium incanum Kunth against the Hemoflagellate Protozoan Trypanosoma cruzi

David Alejandro Hernández-Marín  1 , Rocio Castro-Rios  2 , Abelardo Chávez-Montes  3 , Sandra L Castillo-Hernández  4 , Joel Horacio Elizondo-Luevano  5 , Martín Humberto Muñoz-Ortega  6 , Eduardo Sánchez-García  3
Affiliations

Antiparasitic Activity of Isolated Fractions from Parthenium incanum Kunth against the Hemoflagellate Protozoan Trypanosoma cruzi

David Alejandro Hernández-Marín et al. Antibiotics (Basel). .

Abstract

This study focused on isolating, identifying, and evaluating the trypanocidal potential against the hemoflagellate protozoan Trypanosoma cruzi of compounds from Parthenium incanum (Mariola), a plant used in traditional Mexican medicine to treat stomach and liver disorders. P. incanum has a wide distribution in Mexico. This study found that methanolic extracts of P. incanum, obtained by static maceration and successive reflux, had promising results. The fractions were compared using thin-layer chromatography (TLC) and those that showed similarities were mixed. A bioguided assay was performed with Staphylococcus aureus ATCC 25923, using agar diffusion and bioautography techniques to determine the preliminary biological activity. The fractions with antimicrobial activity were purified using a preparative thin-layer chromatography (PTLC) plate, obtaining the bioactive bandages that were subjected to a trypanocidal evaluation against the Ninoa strain of T. cruzi in its epimastigote stage. This revealed an IC50 of up to 45 ± 2.5 µg/mL, in contrast to the values obtained from the crude extracts of less than 100 µg/mL. The TLC, Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS) techniques were used to identify the compounds, demonstrating the presence of sesquiterpene lactones, parthenin, and coronopolin. We concluded that these compounds have the potential to inhibit T. cruzi growth.

Keywords: FT-IR; HPLC–MS; Parthenium incanum; coronopolin; parthenin; trypanocidal.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflicts of interest. The funders had no role in this study’s design; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

Figures

Figure 1
Figure 1
FT-IR spectra obtained for the (a) maceration-1 and (b) Soxhlet-1 active fractions of Parthenium incanum Kunth.
Figure 2
Figure 2
Total Ion Chromatograms obtained for the (a) maceration-1 and (b) Soxhlet-1 isolated active fractions.
Figure 3
Figure 3
MS/MS spectra using, as precursors, the ions at (a) m/z 263 and (b) m/z 265.
Figure 4
Figure 4
Chemical structures of parthenin and coronopolin.
See this image and copyright information in PMC

References

    1. UNAM/INPI Atlas de las Plantas de la Medicina Tradicional Mexicana. Biblioteca Digital de la Medicina Tradicional Mexicana. 2009. [(accessed on 25 June 2024)]. Available online: http://www.medicinatradicionalmexicana.unam.mx/apmtm/index.html.
    1. Xu Y.M., Wijeratne E.K., Calderon-Rivera A., Loya-López S., Perez-Miller S., Khanna R., Gunatilaka A.L. Argentatin C Analogues with Potential Antinociceptive Activity and Other Triterpenoid Constituents from the Aerial Parts of Parthenium incanum. ACS Omega. 2023;8:20085–20095. doi: 10.1021/acsomega.3c02302. - DOI - PMC - PubMed
    1. Kaur L., Malhi D.S., Cooper R., Kaur M., Sohal H.S., Mutreja V., Sharma A. Comprehensive review on ethnobotanical uses, phytochemistry, biological potential and toxicology of Parthenium hysterophorus L.: A journey from noxious weed to a therapeutic medicinal plant. J. Ethnopharmacol. 2021;281:114525. doi: 10.1016/j.jep.2021.114525. - DOI - PubMed
    1. Mazur M., Masłowiec D. Antimicrobial activity of lactones. Antibiotics. 2022;11:1327. doi: 10.3390/antibiotics11101327. - DOI - PMC - PubMed
    1. Taljaard L., Haynes R.K., Van Der Kooy F. Artemisia Species and Their Active Constituents for Treating Schistosomiasis. Rev. Bras. Farmacogn. 2023;33:875–885. doi: 10.1007/s43450-023-00407-5. - DOI

LinkOut - more resources