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. 2024 Jul 22;14(14):1577.
doi: 10.3390/diagnostics14141577.

Liquid Biopsies in Follicular Thyroid Carcinomas-A Brief Report

Marie-Louise Uhre Hansen  1 , Simone Kloch Bendtsen  1 , Kathrine Kronberg Jakobsen  1 , Ane Yde Schmidt  2 , Christoffer Holst Hahn  1 , Christian von Buchwald  1   3 , Christian Grønhøj  1   3
Affiliations

Liquid Biopsies in Follicular Thyroid Carcinomas-A Brief Report

Marie-Louise Uhre Hansen et al. Diagnostics (Basel). .

Abstract

Thyroid cancer (TC) represents a significant health burden globally, with follicular thyroid cancer (FTC) posing diagnostic challenges despite advancements. This pilot study aimed to evaluate the utility of a liquid biopsy with cell-free DNA (cfDNA) in patients with FTC. Blood samples were collected from 13 patients diagnosed with FTC, DNA extraction was performed, and cfDNA was analyzed using the Illumina's TruSight Oncology 500 High-Throughput panel. The results revealed low tumor mutational burden and minimal pathogenic variants in cfDNA, indicating challenges such as low DNA yield and poor material quality despite adequate coverage. Our findings indicate that cfDNA as an add-on diagnostic tool in patients with FTC might not be a useful supplement.

Keywords: cell-free DNA; follicular thyroid cancer; liquid biopsies.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Flow diagram of study.
Figure 2
Figure 2
Box plot of extracted DNA from plasma (ng) in relation to (A) T-category and (B) N-category. Abbreviation: T-category, primary tumor status; N-category, region lymph node status.
See this image and copyright information in PMC

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