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. 2024 Jul 12;12(7):1414.
doi: 10.3390/microorganisms12071414.

Fecal Microbiota Characteristics in Constipation-Predominant and Mixed-Type Irritable Bowel Syndrome

Mariya Gryaznova  1 , Yuliya Smirnova  1 , Inna Burakova  1 , Polina Morozova  1   2 , Svetlana Lagutina  3 , Pavel Chizhkov  2 , Olga Korneeva  1 , Mikhail Syromyatnikov  1   2
Affiliations

Fecal Microbiota Characteristics in Constipation-Predominant and Mixed-Type Irritable Bowel Syndrome

Mariya Gryaznova et al. Microorganisms. .

Abstract

Background: Irritable bowel syndrome (IBS) is a common condition that affects the lifestyle of patients. It is associated with significant changes in the composition of the gut microbiome, but the underlying microbial mechanisms remain to be fully understood. We study the fecal microbiome of patients with constipation-predominant IBS (IBS-C) and mixed-type IBS (IBS-M).

Methods: We sequenced the V3 region of the 16S rRNA on the Ion Torrent PGM sequencing platform to study the microbiome.

Results: In the patients with IBS-C and IBS-M, an increase in alpha diversity was found, compared to the healthy group, and differences in beta diversity were also noted. At the phylum level, both IBS subtypes showed an increase in the Firmicutes/Bacteroidetes ratio, as well as an increase in the abundance of Actinobacteria and Verrucomicrobiota. Changes in some types of bacteria were characteristic of only one of the IBS subtypes, while no statistically significant differences in the composition of the microbiome were detected between IBS-C and IBS-M.

Conclusions: This study was the first to demonstrate the association of Turicibacter sanguinis, Mitsuokella jalaludinii, Erysipelotrichaceae UCG-003, Senegalimassilia anaerobia, Corynebacterium jeikeium, Bacteroides faecichinchillae, Leuconostoc carnosum, and Parabacteroides merdae with IBS subtypes.

Keywords: 16S rRNA; IBS subtypes; intestinal disorders; microbiome; next-generation sequencing.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Bacterial phylum found in patients.
Figure 2
Figure 2
The most abundant species of bacteria found in patients.
Figure 3
Figure 3
Indicators of alpha diversity of the microbiome of patients with IBS-C and IBS-M and patients without gastrointestinal pathology. * p ≤ 0.05, *** p ≤ 0.001.
Figure 4
Figure 4
Indicator of beta diversity of the microbiome of patients with IBS-C, IBS-M, and patients without gastrointestinal pathology.
Figure 5
Figure 5
Characteristics of the microbiome in patients with IBS-C, *** p ≤ 0.001.
Figure 6
Figure 6
Characteristics of the microbiome in patients with IBS-M, *** p ≤ 0.001.
See this image and copyright information in PMC

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