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. 2024:2844:221-238.
doi: 10.1007/978-1-0716-4063-0_15.

Development of an Haa1-Based Biosensor for Acetic Acid Sensing in Saccharomyces cerevisiae

Affiliations

Development of an Haa1-Based Biosensor for Acetic Acid Sensing in Saccharomyces cerevisiae

Elin Blick et al. Methods Mol Biol. 2024.

Abstract

Transcription factor (TF)-based biosensors are important tools in strain development and screening as they can allow accurate monitoring of intracellular concentrations of a molecule. Acetic acid is one of the main inhibitors in lignocellulosic biomass and a major challenge when using yeast cell factories for biorefinery applications. Thus, developing acetic acid tolerant strains is of great importance. The acetic acid sensing biosensor developed relies on the endogenous Saccharomyces cerevisiae TF Haa1 that upon binding of acetic acid translocates to the nucleus. The acetic acid biosensor can be used as a tool for strain development and evaluation, as well as for screening of acetic acid-producing strains and for dynamic monitoring of acetic acid accumulation. This chapter describes a methodology for developing a TF-based biosensor for acetic acid sensing. Protocols for design considerations, part construction, and characterization procedures are included. The approach can potentially be adapted to any molecule where a suitable TF can be identified.

Keywords: Acetic acid; Biosensor; Haa1; Synthetic promoter; Synthetic transcription factor.

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References

    1. Chaisupa P, Wright RC (2023) State-of-the-art in engineering small molecule biosensors and their applications in metabolic engineering. SLAS Technol 100113
    1. Wang G, Øzmerih S, Guerreiro R et al (2020) Improvement of cis, cis-muconic acid production in Saccharomyces cerevisiae through biosensor-aided genome engineering. ACS Synth Biol 9:634–646 - DOI - PubMed - PMC
    1. Liu D, Sica MS, Mao J et al (2022) A p-coumaroyl-CoA biosensor for dynamic regulation of naringenin biosynthesis in Saccharomyces cerevisiae. ACS Synth Biol 11:3228–3238 - DOI - PubMed - PMC
    1. Lee ME, DeLoache WC, Cervantes B et al (2015) A highly characterized yeast toolkit for modular, multipart assembly. ACS Synth Biol 4:975–986 - DOI - PubMed
    1. Entian KD, Kötter P (2007) 25 yeast genetic strain and plasmid collections. Methods Microbiol 36:629–666 - DOI

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