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. 2024 Jul 1;25(7):2265-2269.
doi: 10.31557/APJCP.2024.25.7.2265.

Biomarkers for Oral Squamous Cell Carcinoma (miR-24, miR-200, and miR-34): Screening and Detection MicroRNA

Naghmeh Bahrami  1   2 , Maryam Pirrafiee  3 , Fatemeh Azadi  3 , Rezvan Azimnejad  3 , Seyedeh Zahra Fotook Kiaei  4 , Amir Jalal Abbasi  2   5 , Mehdi Kazempour Dizaji  6 , Somaieh Lookzadeh  7 , Seyed Mohammad Reza Nejatollahi  8 , Mahya Daustani  9 , Hossein Dargahi  10   11 , Abdolreza Mohamadnia  7   12
Affiliations

Biomarkers for Oral Squamous Cell Carcinoma (miR-24, miR-200, and miR-34): Screening and Detection MicroRNA

Naghmeh Bahrami et al. Asian Pac J Cancer Prev. .

Abstract

Introduction: Oral squamous cell carcinoma (OSCC) includes about 90% of all oral malignant tumors, and most of them are diagnosed in advanced stages. This study investigated the expression changes of miR-24, miR-200, and miR-34 in saliva samples of patients with oral squamous cell carcinoma, for early diagnosis.

Methods: In this study, 30 patients and 30 healthy individuals were selected. After RNA extraction and cDNA synthesis, the expression levels of miR-24, miR-200, and miR-34 in saliva samples were measured and evaluated using the Real-Time PCR technique.

Results: Folding change calculation using 2^(-∆∆ Ct) refers to the relative difference in the expression of the markers of the two groups. The expression level of two biomarkers, miR-200 and miR-34, is decreased in patients compared to healthy people; and the expression level of miR-24 is increased in patients compared to healthy people.

Conclusion: In general, considering the availability and convenience of saliva sample collection for early detection of the disease, this research result can be considered a diagnostic screening test. To further prove the research results, conducting more extensive studies with more samples is recommended.

Keywords: Early Diagnosis; miR-200; miR-24; miR-34; oral squamous cell carcinoma.

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Figures

Figure 1
Figure 1
The Rate of miR-200 Positivity in the Saliva of Patients with OSCC and Healthy People
Figure 2
Figure 2
The Rate of miR-24 Positivity in the Saliva of Patients with OSCC and Healthy People
Figure 3
Figure 3
The Rate of miR-34 Positivity in the Saliva of Patients with OSCC and Healthy People
Figure 4
Figure 4
The Difference in miR-34, miR-200, and miR-24 Genes Expression of Control and Treatment Groups
See this image and copyright information in PMC

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