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. 2024 Jul 26:17:3329-3335.
doi: 10.2147/IJGM.S461613. eCollection 2024.

The Performance of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) Virus Using the Colorimetric Reverse-Transcription Loop Mediated Isothermal Amplification (RT-LAMP) Method in Saliva Specimens of Suspected COVID-19 Patients

Dewi Kartika Turbawaty  1 , Andy Sudjadi  1 , Leni Lismayanti  1 , Tiene Rostini  1 , Verina Logito  1
Affiliations

The Performance of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) Virus Using the Colorimetric Reverse-Transcription Loop Mediated Isothermal Amplification (RT-LAMP) Method in Saliva Specimens of Suspected COVID-19 Patients

Dewi Kartika Turbawaty et al. Int J Gen Med. .

Abstract

Introduction: Corona Virus Disease-19 (COVID-19) is a disease caused by Severe-Acute-Respiratory-Syndrome-Coronavirus-2 (SARS-CoV-2). The most reliable and widely accepted method for diagnosing this infection, despite facing various challenges, is the Reverse Transcription Polymerase Chain Reaction (RT-PCR) method, which utilizes nasopharyngeal swab sample. Reverse-transcription loop mediated isothermal amplification (RT-LAMP) is a simpler nucleic acid amplification method compared to the RT-PCR method. This method has several advantages, including: of amplification at constant temperature, faster results, and potentially greater examination capacity.

Purpose: This study aimed to compare the validity of the RT-LAMP method using saliva specimens with that of the RT-PCR method using nasopharyngeal smears.

Methods: This was an analytical observational study with a cross-sectional design. The participants were inpatients in the COVID-19 special isolation building of Hasan Sadikin General Hospital, Indonesia with a probable (clinical symptoms of covid, but not confirm NAAT examination) or confirmed diagnosis of COVID-19 from September 2021 to February 2022. The inclusion criteria are COVID-19 patients with symptoms, adult subjects, and composite mentions. Patients who were unable to secrete saliva were also excluded.

Results: In total, 118 specimens were collected. The validity test results of the saliva specimens using the RT-LAMP method showed sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV), of 65.5%, 100%, 100%, and 75%, respectively. The results increased in subjects treated between 3 and 7 days after symptom onset ie 73.2%, 100%, 100%, and 82.3%, respectively.

Conclusion: The very strong specificity accompanied by good sensitivity and NPV in the group of subjects treated 3-7 days after the onset of symptoms indicates that the RT-LAMP method using saliva specimens can be an efficient and reliable alternative tool in detecting the SARS-CoV-2 virus.

Keywords: COVID-19; RT-LAMP; RT-PCR; SARS-CoV-2; saliva.

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Conflict of interest statement

Dewi Kartika Turbawaty is an academic author, a clinical pathologist, and the Head of Clinical Pathology Department, staff of the Microbiology and Biomolecular Department, Faculty of Medicine Padjadjaran University, and Dr. Hasan Sadikin General Hospital. Andy Sudjadi is a clinical pathologist. Leni Lismayanti is a clinical pathologist and staff member of the Hematology Department, Faculty of Medicine, Padjadjaran University and Dr. Hasan Sadikin General Hospital. Tiene Rostini is a clinical pathologist and staff member in the Clinical Chemistry Department, Faculty of Medicine, Padjadjaran University, and Dr. Hasan Sadikin General Hospital. Verina Logito is a clinical pathologist and staff member of the Immunoserology Department, Faculty of Medicine, Padjadjaran University and Dr. Hasan Sadikin General Hospital. The authors declare that this research was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest.

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