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. 2024 Oct;61(10):1327-1331.
doi: 10.1007/s00592-024-02337-7. Epub 2024 Jul 31.

Knockout of M-LP/Mpv17L, a newly identified atypical PDE, alleviates diabetic conditions in mice

Reiko Iida  1 , Misuzu Ueki  2 , Toshihiro Yasuda  3
Affiliations

Knockout of M-LP/Mpv17L, a newly identified atypical PDE, alleviates diabetic conditions in mice

Reiko Iida et al. Acta Diabetol. 2024 Oct.
No abstract available

Keywords: Cyclic nucleotide phosphodiesterase; Diabetes; Mpv17-like protein; Streptozotocin.

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Conflict of interest statement

The authors declare that there are no conflicts of interest.

Figures

Fig. 1
Fig. 1
Effects of STZ administration in WT and M-LP/Mpv17L-KO mice. a Changes in blood glucose levels in the WT-control, WT-STZ, KO-control and KO-STZ groups (n = 5). *p < 0.05, **p < 0.005 b Levels of Ins and MafA mRNAs on day 13 after STZ treatment in the pancreas of the WT-control, WT-STZ, KO-control and KO-STZ groups. Expression of mRNA was determined by Q-PCR using β-actin as an internal control. The results are expressed as ratios relative to the value for the WT-control group (n = 4). *p < 0.05, #p < 0.05 for similarly treated KO vs. WT. c Levels of M-LP/Mpv17L mRNAs in the pancreas of the WT-control, WT-STZ, KO-control and KO-STZ groups at 13 days after STZ treatment (n = 4). M-LP/Mpv17L mRNA was not detected in the KO-control and KO-STZ groups. Expression of mRNA was determined by Q-PCR using β-actin as an internal control. The results are expressed as ratios relative to the value for the WT-control group (n = 4). *p < 0.05
Fig. 2
Fig. 2
Pathological changes in pancreatic islet cells on day 13 after STZ treatment a, b Representative images of H&E-stained pancreas sections at 13 days after STZ treatment. c Cross-sectional areas of islet cell nuclei in the WT-control, WT-STZ and KO-STZ mice (n = 20). *p < 0.005
See this image and copyright information in PMC

References

    1. Iida R, Yasuda T, Tsubota E et al (2001) Cloning, mapping, genomic organization, and expression of mouse M-LP, a new member of the peroxisomal membrane protein Mpv17 domain family. Biochem Biophys Res Commun 283:292–296 - DOI - PubMed
    1. Iida R, Ueki M, Yasuda T (2020) Human Mpv17-like protein with a mitigating effect on mtDNA damage is involved in cAMP/PKA signaling in the mitochondrial matrix. Biochim Biophys Acta Mol Cell Res 1867:118792 - DOI - PubMed
    1. Iida R, Ueki M, Yasuda T (2022) Deficiency of M-LP/Mpv17L leads to development of β-cell hyperplasia and improved glucose tolerance via activation of the wnt and TGF-β pathways. Biochim Biophys Acta Mol Basis Dis 1868:166318 - DOI - PubMed
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    1. Diao M, Wu Y, Yang J et al (2022) Identification of Novel Key Molecular signatures in the Pathogenesis of Experimental Diabetic kidney disease. Front Endocrinol 13:843721 - DOI - PMC - PubMed

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