Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Oct;61(10):1327-1331.
doi: 10.1007/s00592-024-02337-7. Epub 2024 Jul 31.

Knockout of M-LP/Mpv17L, a newly identified atypical PDE, alleviates diabetic conditions in mice

Reiko Iida  1 , Misuzu Ueki  2 , Toshihiro Yasuda  3
Affiliations

Knockout of M-LP/Mpv17L, a newly identified atypical PDE, alleviates diabetic conditions in mice

Reiko Iida et al. Acta Diabetol. 2024 Oct.
No abstract available

Keywords: Cyclic nucleotide phosphodiesterase; Diabetes; Mpv17-like protein; Streptozotocin.

PubMed Disclaimer

Conflict of interest statement

The authors declare that there are no conflicts of interest.

Figures

Fig. 1
Fig. 1
Effects of STZ administration in WT and M-LP/Mpv17L-KO mice. a Changes in blood glucose levels in the WT-control, WT-STZ, KO-control and KO-STZ groups (n = 5). *p < 0.05, **p < 0.005 b Levels of Ins and MafA mRNAs on day 13 after STZ treatment in the pancreas of the WT-control, WT-STZ, KO-control and KO-STZ groups. Expression of mRNA was determined by Q-PCR using β-actin as an internal control. The results are expressed as ratios relative to the value for the WT-control group (n = 4). *p < 0.05, #p < 0.05 for similarly treated KO vs. WT. c Levels of M-LP/Mpv17L mRNAs in the pancreas of the WT-control, WT-STZ, KO-control and KO-STZ groups at 13 days after STZ treatment (n = 4). M-LP/Mpv17L mRNA was not detected in the KO-control and KO-STZ groups. Expression of mRNA was determined by Q-PCR using β-actin as an internal control. The results are expressed as ratios relative to the value for the WT-control group (n = 4). *p < 0.05
Fig. 2
Fig. 2
Pathological changes in pancreatic islet cells on day 13 after STZ treatment a, b Representative images of H&E-stained pancreas sections at 13 days after STZ treatment. c Cross-sectional areas of islet cell nuclei in the WT-control, WT-STZ and KO-STZ mice (n = 20). *p < 0.005
See this image and copyright information in PMC

References

    1. Iida R, Yasuda T, Tsubota E et al (2001) Cloning, mapping, genomic organization, and expression of mouse M-LP, a new member of the peroxisomal membrane protein Mpv17 domain family. Biochem Biophys Res Commun 283:292–296 - PubMed
    1. Iida R, Ueki M, Yasuda T (2020) Human Mpv17-like protein with a mitigating effect on mtDNA damage is involved in cAMP/PKA signaling in the mitochondrial matrix. Biochim Biophys Acta Mol Cell Res 1867:118792 - PubMed
    1. Iida R, Ueki M, Yasuda T (2022) Deficiency of M-LP/Mpv17L leads to development of β-cell hyperplasia and improved glucose tolerance via activation of the wnt and TGF-β pathways. Biochim Biophys Acta Mol Basis Dis 1868:166318 - PubMed
    1. Waddleton D, Wu W, Feng Y et al (2008) Phosphodiesterase 3 and 4 comprise the major cAMP metabolizing enzymes responsible for insulin secretion in INS-1 (832/13) cells and rat islets. Biochem Pharmacol 76:884893 - PubMed
    1. Diao M, Wu Y, Yang J et al (2022) Identification of Novel Key Molecular signatures in the Pathogenesis of Experimental Diabetic kidney disease. Front Endocrinol 13:843721 - PMC - PubMed

LinkOut - more resources