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Comparative Study
. 2024 Jan-Dec;95(1):e13973.
doi: 10.1111/asj.13973.

Failure of isoxsuprine HCl to increase sheep fertility after cervical versus laparoscopic AI using chilled semen at different durations

[Article in French]
Bülent Bülbül  1 , Cemal Dayanıklı  2 , Ebru Şengül  2 , Aydın Turan  2 , Muhammet Aksoy  2 , Zekariya Nur  3
Affiliations
Comparative Study

Failure of isoxsuprine HCl to increase sheep fertility after cervical versus laparoscopic AI using chilled semen at different durations

[Article in French]
Bülent Bülbül et al. Anim Sci J. 2024 Jan-Dec.

Abstract

To improve the fertility of cervical artificial insemination (AI) in sheep, we investigated isoxsuprine HCl usage on the cervical passage during cervical AI. We also compared cervical and laparoscopic AI fertility results of using chilled semen at different durations. Semen was collected from rams and diluted as 20 × 106 or 400 × 106 spermatozoa/straw for laparoscopic and cervical AI, respectively, and chilled to 4°C within 2 h. Sheep were inseminated with chilled semen for 8 or 24 h via the laparoscopic or cervical AI method. Moreover, some of the cervical inseminated sheep were injected intramuscularly with 0.5 mg/kg of isoxsuprine HCl 15 min before AI. As a result, the use of isoxsuprine HCl did not affect cervical transit and fertility. In addition, fertility was affected by the storage duration of the semen; laparoscopic AI was more successful than cervical AI in terms of fertility; if cervical AI is performed, the duration between semen collection and AI should be less than 8 h after chilling the semen at 4°C, and if laparoscopic AI is performed, the time between semen collection and insemination can be up to 24 h after chilling the semen at 4°C. Longer storage periods should be studied.

Keywords: cervical artificial insemination; isoxsuprine HCl; laparoscopic artificial insemination; semen storage; sheep.

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References

REFERENCES

    1. Abd El‐Hamid, I. S. (2019). Effect of adding different levels of caffeine in the extender on some biochemical constituents, enzymatic activities and physical characteristics of chilled and frozen ram semen. Reproduction in Domestic Animals, 54, 225–233. https://doi.org/10.1111/rda.13339
    1. Alvarez, M., Anel‐Lopez, L., Boixo, J. C., Chamorro, C., Neila‐Montero, M., Montes‐Garrido, R., de Paz, P., & Anel, L. (2019). Current challenges in sheep artificial insemination: A particular insight. Reproduction in Domestic Animals, 54(Suppl 4), 32–40. https://doi.org/10.1111/rda.13523
    1. Anel, L., Kaabi, M., Abroug, B., Alvarez, M., Anel, E., Boixo, J. C., de la Fuente, L. F., & de Paz, P. (2005). Factors influencing the success of vaginal and laparoscopic artificial insemination in churra ewes: A field assay. Theriogenology, 63, 1235–1247. https://doi.org/10.1016/j.theriogenology.2004.07.001
    1. Ax, R. L., Dally, M., Didion, B. A., Lenz, R. W., Love, C. C., Varner, D. D., Hafez, B., & Bellin, M. E. (1993). Semen evaluation. In E. S. E. Hafez (Ed.), Reproduction in Farm Animals (pp. 365–376). Lea&Febiger.
    1. Bhoi, D. B. (2022). Tocolysis in farm animal reproduction. Journal of Livestock Science, 13, 41–47. https://doi.org/10.33259/JLivestSci.2022.41-47

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