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. 2024 Aug 1;39(1):205.
doi: 10.1007/s10103-024-04159-z.

Photobiomodulation with laser and led on mesenchymal stem cells viability and wound closure in vitro

Ana Paula Ferro  1 , Rinaldo Roberto de Jesus Guirro  2 , Maristela Delgado Orellana  3 , Gil Cunha de Santis  4 , Jayme Adriano Farina Junior  5 , Elaine Caldeira de Oliveira Guirro  2
Affiliations

Photobiomodulation with laser and led on mesenchymal stem cells viability and wound closure in vitro

Ana Paula Ferro et al. Lasers Med Sci. .

Abstract

Mesenchymal stem cells can differentiate into specific cell lineages in the tissue repair process. Photobiomodulation with laser and LED is used to treat several comorbidities, can interfere in cell proliferation and viability, in addition to promoting responses related to the physical parameters adopted. Evaluate and compare the effects of laser and LED on mesenchymal cells, with different energy doses and different wavelengths, in addition to viability and wound closure. Mesenchymal stem cells derived from human adipocytes were irradiated with laser (energy of 0.5 J, 2 J and 4 J, wavelength of 660 nm and 830 nm), and LED (energy of 0.5 J, 2 J and 4 J, where lengths are 630 nm and 850 nm). The wound closure process was evaluated through monitoring the reduction of the lesion area in vitro. Viability was determined by analysis with Hoechst and Propidium Iodide markers, and quantification of viable and non-viable cells respectively Data distributions were analyzed using the Shapiro-Wilk test. Homogeneity was analyzed using Levene's test. The comparison between the parameters used was analyzed using the Two-way ANOVA test. The T test was applied to data relating to viability and lesion area. For LED photobiomodulation, only the 630 nm wavelength obtained a significant result in 24, 48 and 72 h (p = 0,027; p = 0,024; p = 0,009). The results related to the in vitro wound closure test indicate that both photobiomodulation with laser and LED demonstrated significant results considering the time it takes to approach the edges (p < 0.05). Considering the in vitro experimental conditions of the study, it is possible to conclude that the physical parameters of photobiomodulation, such as energy and wavelength, with laser or LED in mesenchymal stem cells, can play a potential role in cell viability and wound closure.

Keywords: Low-level light therapy; Mesenchymal stem cells; Photobiomodulation; Semiconductor; Wound healing.

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