Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2024 Sep;44(3):479-493.
doi: 10.1016/j.cll.2024.04.009. Epub 2024 Jun 8.

Flow Cytometry-based Immune Phenotyping of T and B Lymphocytes in the Evaluation of Immunodeficiency and Immune Dysregulation

Alan A Nguyen  1 , Craig D Platt  2
Affiliations
Review

Flow Cytometry-based Immune Phenotyping of T and B Lymphocytes in the Evaluation of Immunodeficiency and Immune Dysregulation

Alan A Nguyen et al. Clin Lab Med. 2024 Sep.

Abstract

There are approximately 500 congenital disorders that impair immune cell development and/or function. Patients with these disorders may present with a wide range of symptoms, including increased susceptibility to infection, autoimmunity, autoinflammation, lymphoproliferation, and/or atopy. Flow cytometry-based immune phenotyping of T and B lymphocytes plays an essential role in the evaluation of patients with these presentations. In this review, we describe the clinical utility of flow cytometry as part of a comprehensive evaluation of immune function and how this testing may be used as a diagnostic tool to identify underlying aberrant immune pathways, monitor disease activity, and assess infection risk.

Keywords: Flow cytometry; Immune dysregulation; Inborn errors of immunity; Primary immunodeficiency.

PubMed Disclaimer

Conflict of interest statement

Disclosure A. A. Nguyen is a staff physician at Boston Children’s Hospital and Instructor of Pediatrics at Harvard Medical School. C. D. Platt is a staff physician and Medical Director of Flow Cytometry at Boston Children’s Hospital, and Assistant Professor of Pediatrics at Harvard Medical School. The spouse of C. D. Platt is employed by Quest Diagnostics. A. A. Nguyen has nothing to disclose.

MeSH terms

LinkOut - more resources