Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Sep:148:102549.
doi: 10.1016/j.tube.2024.102549. Epub 2024 Jul 25.

Detection of Mycobacterium tuberculosis transrenal DNA in urine samples among adults in Peru

Annelies W Mesman  1 , Roger I Calderon  2 , Laura Hauns  3 , Nira R Pollock  4 , Milagros Mendoza  5 , Rebecca C Holmberg  6 , Molly F Franke  7
Affiliations

Detection of Mycobacterium tuberculosis transrenal DNA in urine samples among adults in Peru

Annelies W Mesman et al. Tuberculosis (Edinb). 2024 Sep.

Abstract

Diagnosis of pulmonary tuberculosis (TB) relies on a sputum sample, which cannot be obtained from all symptomatic individuals. Mycobacterium tuberculosis (Mtb) transrenal DNA (trDNA) has been detected in urine, an easily obtainable, noninvasive, alternative sample type. However, reported sensitivities have been variable and likely depend on collection and assay procedures and aspects of trDNA biology. We analyzed three serial urine samples from each of 75 adults with culture-confirmed pulmonary TB disease in Lima, Peru for detection of trDNA using short-fragment real-time PCR. Additionally, we examined host, urine, and sampling factors associated with detection. Overall per-sample sensitivity was 38 % (95 % Confidence Interval [CI] 30-45 %). On an individual level (i.e., any of the three samples positive), sensitivity was 73 % (95 % CI: 62-83 %). Sensitivity was highest among samples from patients with smear-positive TB, 92 % (95 % CI: 62-100 %). Specificity from a single sample from each of 10 healthy controls was 100 % (95 % CI: 69-100 %). Adjusting our assay positivity threshold increased individual-level sensitivity to 88 % (95 % CI: 78-94 %) overall without affecting the specificity. We did not find associations between Mtb trDNA detection and individual characteristics or urine sample characteristics. Overall, our results support the potential of trDNA detection for TB diagnosis.

Keywords: Cell-free DNA; Diagnosis; Diagnostics; Tuberculosis.

PubMed Disclaimer

Conflict of interest statement

Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Molly Franke reports financial support was provided by National Institutes of Health. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Update of

References

    1. Global Tuberculosis Report 2022. https://www.who.int/teams/global-tuberculosis-programme/tb-reports/globa....
    1. Getahun H, Harrington M, O’Brien R & Nunn P Diagnosis of smear-negative pulmonary tuberculosis in people with HIV infection or AIDS in resource-constrained settings: informing urgent policy changes. Lancet 369, 2042–2049 (2007). - PubMed
    1. Dorman SE Diagnosis of HIV-associated tuberculosis. Curr. Opin. HIV AIDS 13, 462–468 (2018). - PubMed
    1. Huerga H et al. Performance of the 2007 WHO Algorithm to Diagnose Smear-Negative Pulmonary Tuberculosis in a HIV Prevalent Setting. PLoS One 7, e51336. (2012). - PMC - PubMed
    1. Starke JR Pediatric tuberculosis: time for a new approach. Tuberculosis 83, 208–12 (2003). - PubMed

MeSH terms

LinkOut - more resources