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. 2024 Aug 5;56(10):1561-1565.
doi: 10.3724/abbs.2024121.

Evolutionary analysis of paired box gene family and biological function exploration of Lr. Pax7 in lamprey ( Lethenteron reissneri)

Ayqeqan Nurmamat  1   2 , Zihao Yan  1   2 , Yao Jiang  1 , Haoran Guan  1   2 , Ruyu Zhuang  1   2 , Shuyuan Zhang  1   2 , Yuesi Zhou  1   2 , Min Xiu  1   2 , Ya Pang  1   2 , Ding Li  1   2 , Liang Zhao  1   2 , Xin Liu  1   2   3 , Yinglun Han  1   2   3
Affiliations

Evolutionary analysis of paired box gene family and biological function exploration of Lr. Pax7 in lamprey ( Lethenteron reissneri)

Ayqeqan Nurmamat et al. Acta Biochim Biophys Sin (Shanghai). .
No abstract available

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

None
Figure 1
Bioinformatics analysis The identification and sequence analysis of Pax family genes. (A) Protein sequence alignment of the Hm. Pax7-Bl. Pax7. (B) Phylogenetic tree for the Pax family based on the NJ method. (C) Prediction of the tertiary structures of Pax7, Pax6, Pax2, and Pax9 in humans and lampreys. (D) Comparison of the domains between Pax1-Pax9 from humans and lampreys. (E) Diverse genomic synteny analysis of the Hm. Pax7 to Lr. Pax7. (F) Motif composition of the Pax7 family proteins. The full motif composition of the Pax family and the full names of the species are listed in Supplementary Table 1, and the matching amino acid sequences in the motifs are listed in Supplementary Table 2.
None
Figure 2
Expression of Lr. Pax7 in tissues and analysis of Lr. Pax7 gene silencing transcriptome data (A) Relative Lr.Pax7 mRNA expression levels in various lamprey tissues. **** P<0.001 (paired t-test). (B) Western blot analysis of the protein expression levels of Lr.Pax7 in different tissues. (C) The expression of Lr. Pax7 gene was quantified using qPCR following the silencing of Lr. Pax7 gene, and an abundance map was generated. Lr. GAPDH served as the reference gene, and the results are expressed as the means±SD. *Indicates significant difference compared with the control group ( n=3, ns: not significant, *** P<0.005 (paired t-test). (D) Bar chart illustrating the relative expression levels of differentially expressed genes in the Lr. Pax7 transcriptome using qPCR. Ns denotes non-significant, * P<0.05, ** P<0.01, and **** P<0.001 (paired t-test). (E–G) GO enrichment analysis results for the gene silencing expression in the transcriptome. (E) Total genes regulated by Lr. Pax7 gene silencing. (F) Up-regulated genes by Lr. Pax7 gene silencing. (G) Down-regulated genes by Lr. Pax7 gene silencing. (H–J) KEGG enrichment analysis results for the gene silencing expression in the transcriptome. (H) Lr. Pax7 gene silencing: total regulated genes. (I) Down-regulated genes by Lr. Pax7 gene silencing. (J) KEGG pathway classification.
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