[Method of embedding cultures in situ in epoxy resins for comprehensive light and electron microscopic analysis]

Abstract

A simple method for making preparations for light and electron microscopy analysis both from tissue cultures and other biological objects is proposed. The techniques of in situ flat-parallel embedding in special forms between two layers of polyethylene phthalate (PEPh) film are described. The method makes the maximum use of all the properties of the PEPh film allowing to utilize it as a substrate-support for growing cultures and as a substrate for forming optical properties of the preparations. The preparations so made are suitable for preliminary examinations by light microscopy, photography, and marking the selected areas for subsequent flat and sagital ultratomy.