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. 2024 Aug;29(32):2400486.
doi: 10.2807/1560-7917.ES.2024.29.32.2400486.

Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024

Leonard Schuele  1   2 , Leandre Murhula Masirika  2   3   4   5 , Jean Claude Udahemuka  2   6   7 , Freddy Belesi Siangoli  8 , Justin Bengehya Mbiribindi  8 , Pacifique Ndishimye  7   9 , Frank M Aarestrup  10 , Marion Koopmans  1 , Bas B Oude Munnink  1 , Richard Molenkamp  1 ; GREATLIFE MPOX group  11 ; Collaborators
Collaborators, Affiliations

Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024

Leonard Schuele et al. Euro Surveill. 2024 Aug.

Abstract

Monkeypox virus (MPXV) is an emerging zoonotic pathogen with complex epidemiology necessitating rapid diagnosis and distinguishing between clades and subclades. The emerging Clade Ib lacks the genomic region used in the Clade I-specific assay from the Centers for Disease Control and Prevention. We report an MPXV real-time PCR to specifically detect Clade Ib. The assay demonstrated proficient sensitivity and specificity in 92 samples and can be included along other TaqMan-based assays to detect MPXV and distinguish between clades and subclades.

Keywords: Monkeypox virus; Monkeypox virus Clade Ib; Mpox virus Clade Ib; Real-time PCR; diagnostic test.

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Conflict of interest statement

Conflict of interest: None declared.

Figures

Figure
Figure
Alignment of primers and probes with monkeypox virus sequences, September 2023–May 2024
See this image and copyright information in PMC

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