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. 2024 Aug 2;14(15):1673.
doi: 10.3390/diagnostics14151673.

Nasal Microbiome in Granulomatosis with Polyangiitis Compared to Chronic Rhinosinusitis

Eliza Brożek-Mądry  1 , Zofia Burska  1 , Katarzyna Życińska  2 , Janusz Sierdziński  3
Affiliations

Nasal Microbiome in Granulomatosis with Polyangiitis Compared to Chronic Rhinosinusitis

Eliza Brożek-Mądry et al. Diagnostics (Basel). .

Abstract

Rhinosinusitis in granulomatosis with polyangiitis (GPA) is categorised as a secondary, diffuse and inflammatory chronic rhinosinusitis (CRS). It is one of the conditions that impacts the nasal microbiota. This study aimed to compare the nasal microbiomes of patients with GPA, CRS and NSP. A total of 31 patients were included in the study (18 GPA, 6 CRS and 7 nasal septum perforation (NSP)). In all patients, SNOT 22, a nasal endoscopy (Lund-Kennedy scale) and a brush swab were performed. The metagenomic analysis was carried out based on the hypervariable V3-V4 region of the 16S rRNA gene. At the genus level, statistically significant differences were observed in two comparisons: the GPA/NSP and the GPA/CRS groups. In the GPA/NSP group, the differences were related to four genera (Actinomyces, Streptococcus, Methylobacterium-Methylorubrum, Paracoccus), while in the GPA/CRS group, they were related to six (Kocuria, Rothia, Cutibacterium, Streptococcus, Methylobacterium-Methylorubrum, Tepidimonas). Patients with GPA had lower diversity compared to CRS and NSP patients. There were no statistically significant differences found for the Staphylococcus family and Staphylococcus aureus between the three groups.

Keywords: chronic rhinosinusitis; granulomatosis with polyangiitis; microbiome; nasal septal perforation.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Taxonomic ranks in GPA patients with 3 most abundant genera marked. I–VI—incidence of phyla starting with I—most common.
Figure 2
Figure 2
Most abundant bacteria in GPA group at genus level.
Figure 3
Figure 3
Taxonomic ranks in CRS patients with 3 most abundant genera marked. I–III—incidence of phyla starting with I—most common (ex aequo Firmicutes and Actinobacteriota).
Figure 4
Figure 4
Most abundant bacteria in CRS group at genus level.
Figure 5
Figure 5
Taxonomic ranks in NSP patients with 3 most abundant genera marked. I–III—incidence of phyla starting with I—most common.
Figure 6
Figure 6
Most abundant bacteria in NSP group at genus level.
Figure 7
Figure 7
Alpha diversity in the 3 analysed groups—GPA, NSP and CRS.
See this image and copyright information in PMC

References

    1. Kumpitsch C., Koskinen K., Schöpf V., Moissl-Eichinger C. The Microbiome of the Upper Respiratory Tract in Health and Disease. BMC Biol. 2019;17:87. doi: 10.1186/s12915-019-0703-z. - DOI - PMC - PubMed
    1. Dimitri-Pinheiro S., Soares R., Barata P. The Microbiome of the Nose-Friend or Foe? Allergy Rhinol. 2020;11:2152656720911605. doi: 10.1177/2152656720911605. - DOI - PMC - PubMed
    1. Psaltis A.J., Wormald P.-J. Therapy of Sinonasal Microbiome in CRS: A Critical Approach. Curr. Allergy Asthma Rep. 2017;17:59. doi: 10.1007/s11882-017-0726-x. - DOI - PubMed
    1. De Boeck I., van den Broek M.F.L., Allonsius C.N., Spacova I., Wittouck S., Martens K., Wuyts S., Cauwenberghs E., Jokicevic K., Vandenheuvel D., et al. Lactobacilli Have a Niche in the Human Nose. Cell Rep. 2020;31:107674. doi: 10.1016/j.celrep.2020.107674. - DOI - PubMed
    1. Yamanishi S., Pawankar R. Current Advances on the Microbiome and Role of Probiotics in Upper Airways Disease. Curr. Opin. Allergy Clin. Immunol. 2020;20:30–35. doi: 10.1097/ACI.0000000000000604. - DOI - PubMed

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