Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Sep;67(3):273-278.
doi: 10.1637/aviandiseases-D-23-00031.

Cross-Protection Conferred by Combined Vaccine Containing Infectious Bronchitis Virus Attenuated Massachusetts and Recombinant LaSota Virus Expressing Arkansas Spike

R Espejo  1 , C Breedlove  1 , L F da Silva  2 , K Joiner  1 , H Toro  3
Affiliations

Cross-Protection Conferred by Combined Vaccine Containing Infectious Bronchitis Virus Attenuated Massachusetts and Recombinant LaSota Virus Expressing Arkansas Spike

R Espejo et al. Avian Dis. 2023 Sep.

Abstract
in English, Spanish

We previously demonstrated that a prime-boost regime with an infectious bronchitis virus (IBV) Massachusetts (Mass)-type vaccine and recombinant LaSota virus (rLS) coexpressing IBV Arkansas (Ark)-type trimeric spike ectodomain (Se) and granulocyte macrophage colony stimulating factor (GMCSF) enhances heterologous protection against virulent Ark challenge. This study evaluates protection against Ark-type challenge conferred by administering the rLS/ArkSe.GMCSF and the attenuated Mass viruses mixed in the same vial as a combined vaccine. Chickens were vaccinated at day of hatch and challenged at 21 days of age with virulent Ark. Protection conferred by vaccination was assessed by respiratory signs, tracheal virus isolation as well as IBV RNA quantitation, and tracheal histomorphometry. Protection conferred by the combined vaccine was compared to protection induced by a commercial attenuated ArkDPI (Delmarva Poultry Industry) vaccine as well as by the attenuated Mass vaccine alone. Vaccination with the combined vaccine (rLS/ArkSe.GMCSF + Mass) as well as Mass alone provided significantly less protection against Ark challenge compared to the control using attenuated live ArkDPI vaccine. Only ArkDPI-vaccinated chickens exhibited "sterilizing immunity," i.e., no virus isolated from ≥10% of chickens after challenge. Chickens vaccinated with the combined vaccine rLS/ArkSe.GMCSF + Mass showed significantly less tracheal damage than birds vaccinated with the attenuated Mass vaccine alone. In addition, the combined vaccine also resulted in less virus isolation from the trachea. We concluded that the combined vaccine containing the recombinant virus and the attenuated Mass enhanced the cross-protective ability of the attenuated Mass vaccine against heterologous challenge.

Nota de investigación- Protección cruzada conferida por una vacuna combinada que contiene el virus atenuado de la bronquitis infecciosa serotipo Massachusetts y un virus de Newcastle LaSota recombinante que expresa la proteína de la espícula del serotipo Arkansas. Previamente se demostró que un esquema de refuerzo con una vacuna tipo Massachusetts (Mass) del virus de la bronquitis infecciosa (IBV) y con un virus de Newcastle LaSota recombinante (rLS) que co-expresa el ectodominio de pico trimérico del serotipo Arkansas (Ark) del virus de la bronquitis infecciosa (Se) y el factor de estimulación de colonias de granulocitos y macrófagos (GMCSF) (virus rLS/ArkSe.GMCSF) mejora la protección heteróloga contra el desafío con serotipo Arkansas virulento. Este estudio evaluó la protección contra el desafío con el serotipo Arkansas conferida por la administración del virus recombinante rLS/ArkSe.GMCSF y el virus Massachussets atenuado mezclados en el mismo vial como una vacuna combinada. Los pollos se vacunaron el día de la eclosión y se desafiaron a los 21 días de edad con el serotipo Arkansas virulento. La protección conferida por la vacunación se evaluó mediante signos respiratorios, el aislamiento del virus traqueal, cuantificación del ARN del virus de bronquitis y por histomorfometría traqueal. La protección conferida por la vacuna combinada se comparó con la protección inducida por una vacuna comercial atenuada ArkDPI (Delmarva Poultry Industry), y con la vacuna atenuada Massachussets por si sola. La vacunación con la vacuna combinada (rLS/ArkSe.GMCSF + Mass), así como con Massachussets sola, proporcionó una protección significativamente menor contra la exposición con Arkansas en comparación con el control que utilizó la vacuna ArkDPI viva atenuada. Solo los pollos vacunados con ArkDPI exhibieron “inmunidad esterilizante”, es decir, que no se aisló ningún virus en ≥10 % de los pollos después del desafío. Los pollos vacunados con la vacuna combinada rLS/ArkSe.GMCSF + Mass mostraron significativamente menos daño traqueal que las aves vacunadas con la vacuna Massachusetts atenuada sola. Además, la vacuna combinada también resultó en un menor aislamiento del virus de la tráquea. Se concluye que la vacuna combinada que contiene el virus recombinante y el serotipo Massachussets atenuado mejoró la capacidad de protección cruzada de la vacuna de Massachusetts atenuada contra el desafío heterólogo.

Keywords: LaSota; Newcastle virus; chicken; coronavirus; infectious bronchitis virus; poultry diseases; poultry vaccine; recombinant; vaccine.

PubMed Disclaimer

References

    1. Kusters JG, Jager EJ, Niesters HG, van der Zeijst BA. Sequence evidence for RNA recombination in field isolates of avian coronavirus infectious bronchitis virus. Vaccine 8:605–608; 1990.
    1. Cavanagh D, Davis PJ, Cook JK. Infectious bronchitis virus: evidence for recombination within the Massachusetts serotype. Avian Pathol. 21:401–408; 1992.
    1. Wang L, Junker D, Collisson EW. Evidence of natural recombination within the S1 gene of infectious bronchitis virus. Virology 192:710–716; 1993.
    1. Jia W, Karaca K, Parrish CR, Naqi SA. A novel variant of avian infectious bronchitis virus resulting from recombination among three different strains. Arch Virol. 140:259–271; 1995.
    1. Kottier SA, Cavanagh D, Britton P. Experimental evidence of recombination in coronavirus infectious bronchitis virus. Virology 213:569–580; 1995.

Publication types

MeSH terms

LinkOut - more resources