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The genome sequence of the Judas Tree Seed Beetle, Bruchidius siliquastri Delobel, 2007

Maxwell V L Barclay et al. Wellcome Open Res. .

Abstract

We present a genome assembly from an individual male Bruchidius siliquastri (the Judas tree Seed Beetle; Arthropoda; Insecta; Coleoptera; Chrysomelidae). The genome sequence is 375.6 megabases in span. Most of the assembly is scaffolded into 11 chromosomal pseudomolecules, including the X and Y sex chromosomes. The mitochondrial genome has also been assembled and is 15.81 kilobases in length. Gene annotation of this assembly on Ensembl identified 17,940 protein coding genes.

Keywords: Bruchidius siliquastri; Coleoptera; chromosomal; genome sequence; the Judas tree Seed Beetle.

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Conflict of interest statement

No competing interests were disclosed.

Figures

Figure 1.
Figure 1.
Photographs of Bruchidius siliquastri: a) A specimen collected in Kensington in 2014: photograph by Harry Taylor (Natural History Museum); b)– d) The specimen used for genome sequencing (icBruSili1): b) dorsal view, c) lateral view and d) ventral view.
Figure 2.
Figure 2.. Genome assembly of Bruchidius siliquastri, icBruSili1.1: metrics.
The BlobToolKit Snailplot shows N50 metrics and BUSCO gene completeness. The main plot is divided into 1,000 size-ordered bins around the circumference with each bin representing 0.1% of the 375,583,928 bp assembly. The distribution of scaffold lengths is shown in dark grey with the plot radius scaled to the longest scaffold present in the assembly (49,182,353 bp, shown in red). Orange and pale-orange arcs show the N50 and N90 scaffold lengths (39,857,073 and 26,473,722 bp), respectively. The pale grey spiral shows the cumulative scaffold count on a log scale with white scale lines showing successive orders of magnitude. The blue and pale-blue area around the outside of the plot shows the distribution of GC, AT and N percentages in the same bins as the inner plot. A summary of complete, fragmented, duplicated and missing BUSCO genes in the endopterygota_odb10 set is shown in the top right. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/CASGFI01/dataset/CASGFI01/snail.
Figure 3.
Figure 3.. Genome assembly of Bruchidius siliquastri, icBruSili1.1: BlobToolKit GC-coverage plot.
Scaffolds are coloured by phylum. Circles are sized in proportion to scaffold length. Histograms show the distribution of scaffold length sum along each axis. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/CASGFI01/dataset/CASGFI01/blob.
Figure 4.
Figure 4.. Genome assembly of Bruchidius siliquastri, icBruSili1.1: BlobToolKit cumulative sequence plot.
The grey line shows cumulative length for all scaffolds. Coloured lines show cumulative lengths of scaffolds assigned to each phylum using the buscogenes taxrule. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/CASGFI01/dataset/CASGFI01/cumulative.
Figure 5.
Figure 5.. Genome assembly of Bruchidius siliquastri, icBruSili1.1: Hi-C contact map of the icBruSili1.1 assembly, visualised using HiGlass.
Chromosomes are shown in order of size from left to right and top to bottom. An interactive version of this figure may be viewed at https://genome-note-higlass.tol.sanger.ac.uk/l/?d=ff3I2oGrSVaZWymS1fyS7w.
See this image and copyright information in PMC

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