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. 1985 Dec;151(2):263-7.
doi: 10.1016/0003-2697(85)90174-5.

Method of enzymatic determination of pyrroloquinoline quinone

M Ameyama, M Nonobe, E Shinagawa, K Matsushita, O Adachi

Method of enzymatic determination of pyrroloquinoline quinone

M Ameyama et al. Anal Biochem. 1985 Dec.

Abstract

An improved enzymatic method for the determination of pyrroloquinoline quinone, a novel prosthetic group of some important oxidoreductases, has been developed with cytoplasmic membrane of Escherichia coli K-12, in which D-glucose dehydrogenase (EC 1.1.99.17) was completely resolved to apo-enzyme by EDTA treatment. Incubation of the EDTA-treated membrane with exogenous pyrroloquinoline quinone in the presence of magnesium ions gave a quantitative determination of pyrroloquinoline quinone by assaying the restored D-glucose dehydrogenase activity. This novel enzymatic method was confirmed to be highly reproducible up to 10 ng of pyrroloquinoline quinone and could be applied to a routine assay of pyrroloquinoline quinone.

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