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. 1985 Dec;151(2):431-7.
doi: 10.1016/0003-2697(85)90200-3.

Preparative purification of Escherichia coli heat-stable enterotoxin

A M Saeed, R N Greenberg

Preparative purification of Escherichia coli heat-stable enterotoxin

A M Saeed et al. Anal Biochem. 1985 Dec.

Abstract

Heat-stable enterotoxin (STa) isolated from bovine Escherichia coli strains was purified to homogeneity by growing the bacterial strains in a chemically defined medium, desalting, and concentrating the culture filtrate by batch adsorption chromatography on Amberlite XAD-2 resin, batch adsorption chromatography on reversed-phase silica, and preparative reversed-phase high-performance liquid chromatography. This rapid preparative purification scheme gave high recovery yields of pure STa which exhibited biochemical homology to STa purified by more complicated procedures.

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