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. 2024 Jul 31:37:13178.
doi: 10.3389/ti.2024.13178. eCollection 2024.

Orthostatic Ex-Vivo Lung Perfusion (EVLP): A Proof of Concept

Massimo Boffini  1 , Andrea Costamagna  2 , Matteo Marro  1 , Erika Simonato  1 , Paola Cassoni  3 , Luca Bertero  3 , Vito Fanelli  2 , Cristina Barbero  1 , Luca Brazzi  2 , Mauro Rinaldi  1
Affiliations

Orthostatic Ex-Vivo Lung Perfusion (EVLP): A Proof of Concept

Massimo Boffini et al. Transpl Int. .

Abstract

The key goal in lung donation remains the improvement of graft preservation with the ultimate objective of increasing the number and quality of lung transplants (LTx). Therefore, in recent years the field of graft preservation focused on improving outcomes related to solid organ regeneration and restoration. In this contest Ex-Vivo Lung Perfusion (EVLP) plays a crucial role with the purpose to increase the donor pool availability transforming marginal and/or declined donor lungs suitable for transplantation. Aim of this proof of concept is to test the safety, suitability and feasibility of a new tilting dome for EVLP designed considering the dorsal lung areas as the "Achilles' heel" of the EVLP due to a more fluid accumulation than in the supine standard position.

Keywords: EVLP; animal model; lung transplant; organ preservation; perfusion.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Lung block positioned in the dome with different degree of inclination.
FIGURE 2
FIGURE 2
Time course of the delta PaO2/FiO2, Cdyn and Cstat variables.
FIGURE 3
FIGURE 3
Timeline of the procedure.
FIGURE 4
FIGURE 4
1. Hematoxylin and eosin stain analysis of the whole block at the end of the perfusion. (A): upper right lobe; (B): medium right lobe; (C): lower right lobe; (D): upper left lobe; (E): accessory left lobe; (F): lower left lobe; 2. Hematoxylin and eosin stain analysis at different time of perfusion: (A): T0; (B): T1; (C): T2; (D): T3.
See this image and copyright information in PMC

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