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Review
. 2024 Nov;47(6):1393-1403.
doi: 10.1080/01480545.2024.2367561. Epub 2024 Aug 19.

Toxicological assays in the evaluation of safety assessment of fibrinolytic enzymes

Affiliations
Review

Toxicological assays in the evaluation of safety assessment of fibrinolytic enzymes

Marllyn Marques da Silva et al. Drug Chem Toxicol. 2024 Nov.

Abstract

Cardiovascular diseases (CVDs) cause 30% of deaths each year, and in 2030, around 23.6 million people will die due to CVDs. The major challenge is to obtain molecules with minimal adverse reactions that can prevent and dissolve blood clots. In this context, fibrinolytic enzymes from diverse microorganism sources have been extensively investigated due to their potential to act directly and specifically on the fibrin clot, preventing side effects and performing potential thrombolytic effects. However, most researches focus on the purification and characterization of proteases, with little emphasis on the mechanism of action and pharmacological characteristics, including toxicity assays which are essential to assess safety and side effects. Therefore, this work aims to emphasize the importance of evaluations indicating the toxicological profile of fibrinolytic proteases through in vitro and in vivo tests. Both types of assays contribute as preclinical stage in drug development and are crucial for clinical applications. This scarcity creates arbitrary barriers to further studies. This work should further encourage the development of studies to ensure the safety and effectivity of fibrinolytic proteases.

Keywords: Toxicity; biocompatibility; biological applications; proteases; thrombolytic therapy.

Plain language summary

Suggested pre-clinical trials aim to validate more specific methods for fibrinolytic enzymes;Current toxicity standards can be adapted to better assess the profile of fibrinolytic enzymes;The class of fibrinolytic enzymes must be carefully evaluated according to the method of application.

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