Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Oct 8;68(10):e0073424.
doi: 10.1128/aac.00734-24. Epub 2024 Aug 21.

Use of frozen native feces for fecal microbiota transplantation in recurrent Clostridioides difficile infection: a simple way to improve the efficiency of donor feces preparation

Rachel Sintes  1   2   3   4 , Paul McLellan  2   3   4   5 , Gabriele Navelli  1   2   3   4 , Cécilia Landman  2   3   4   5 , Sandrine Delage  1   2   3   4 , Sandrine Truong  2   3   4   5 , Nicolas Benech  4   6 , Nathalie Kapel  2   3   4   7 , Alicia Moreino Sabater  2   3   4   8 , Aurélie Schnuriger  2   3   4   9 , Catherine Eckert  2   3   4   10 , Alexandre Bleibtreu  2   3   4   11 , Anne-Christine Joly  1   2   3   4 , Harry Sokol  2   3   4   5   12
Affiliations

Use of frozen native feces for fecal microbiota transplantation in recurrent Clostridioides difficile infection: a simple way to improve the efficiency of donor feces preparation

Rachel Sintes et al. Antimicrob Agents Chemother. .

Abstract

Preparing fecal microbiota transplants immediately after donation is resource-intensive, and a proportion are destroyed following abnormal screening results. We retrospectively compared two processes, frozen fecal preparation (FFP) and fresh native frozen preparation (FNFP), for clinical efficacy in the treatment of recurrent Clostridioides difficile infection (rCDI). FFP and FNFP were similarly effective with clinical success rates of 76.7% and 86.7% (P = 0.32), respectively. FNFP is an efficient procedure that saves resources while maintaining clinical efficacy in rCDI.

Keywords: Clostridium difficile; FMT; fecal microbiota transplantation.

PubMed Disclaimer

Conflict of interest statement

H.S. reports lecture fee, board membership, or consultancy from Amgen, Fresenius, IPSEN, Actial, Astellas, Danone, THAC, Biose, BiomX, Eligo, Immusmol, Adare, Nestle, Ferring, MSD, Bledina, Pfizer, Biocodex, BMS, Bromatech, Gilead, Janssen, Mayoli, Roche, Sanofi, Servier, Takeda, Abbvie, and Lilly, has stocks from Enterome bioscience, and is co-founder of Exeliom Biosciences.

Figures

Fig 1
Fig 1
Clinical success of the two fecal preparation procedures. (A) Frozen fecal preparation (FFP), (B) fresh native frozen stool preparation (FNFP), (C) clinical success of FMT, and (D) clinical success of FMT restricted to donor D7.
See this image and copyright information in PMC

References

    1. van Nood E, Vrieze A, Nieuwdorp M, Fuentes S, Zoetendal EG, de Vos WM, Visser CE, Kuijper EJ, Bartelsman J, Tijssen JGP, Speelman P, Dijkgraaf MGW, Keller JJ. 2013. Duodenal infusion of donor feces for recurrent Clostridium difficile. N Engl J Med 368:407–415. doi:10.1056/NEJMoa1205037 - DOI - PubMed
    1. Ansm . 2016. La transplantation de microbiote fécal et son encadrement dans les essais cliniques
    1. Trang C, Scanzi J, Galperine T, Mosca A, Batista R, Sokol H. 2017. Transplantation de microbiote fécal dans le cadre des infections à Clostridium difficile récidivantes: actualisation des recommandations pour la pratique clinique courante. Hepato-Gastro Oncol Dig 24:319–325. doi:10.1684/hpg.2017.1438 - DOI
    1. Youngster I, Sauk J, Pindar C, Wilson RG, Kaplan JL, Smith MB, Alm EJ, Gevers D, Russell GH, Hohmann EL. 2014. Fecal microbiota transplant for relapsing Clostridium difficile infection using a frozen inoculum from unrelated donors: a randomized, open-label, controlled pilot study. Clin Infect Dis 58:1515–1522. doi:10.1093/cid/ciu135 - DOI - PMC - PubMed
    1. Hamilton MJ, Weingarden AR, Sadowsky MJ, Khoruts A. 2012. Standardized frozen preparation for transplantation of fecal microbiota for recurrent Clostridium difficile infection. Am J Gastroenterol 107:761–767. doi:10.1038/ajg.2011.482 - DOI - PubMed

Publication types

LinkOut - more resources