Mitigating N2O emissions in land treatment systems: Mechanisms, influences, and future directions

Abstract

Land treatment systems (LTS) are widely used in decentralized domestic wastewater treatment due to low energy requirements and effective treatment outcomes. However, LTS operations are also a significant source of N₂O emissions, a potent greenhouse gas threatening the ozone layer and posing risks to human health. Despite the importance of understanding and controlling N₂O emissions, existing literature lacks comprehensive analyses of the mechanisms driving N₂O generation and effective control strategies within LTS. This study addresses this gap by reviewing current research and identifying key factors influencing N₂O emissions in LTS. This review reveals that in addition to traditional nitrification and denitrification processes, co-denitrification and complete ammonia oxidation are crucial for microbial nitrogen removal in LTS. Plant selection is primarily based on their nitrogen absorption capacity while using materials such as biochar and iron can provide carbon sources or electrons to support microbial activities. Optimizing operational parameters is essential for reducing N₂O emissions and enhancing nitrogen removal efficiency in LTS. Specifically, the carbon-to‑nitrogen ratio should be maintained between 5 and 12, and the hydraulic loading rate should be kept within 0.08-0.2 m³/(m²·d). Dissolved oxygen and oxidation-reduction potential should be adjusted to meet the aerobic or anaerobic conditions the microorganisms require. Additionally, maintaining a pH range of 6.5-7.5 by adding alkaline substances is crucial for sustaining nitrous oxide reductase activity. The operating temperature should be maintained between 20 and 30 °C to support optimal microbial activity. This review further explores the relationship between environmental factors and microbial enzyme activity, community structure changes, and functional gene expression related to N₂O production. Future research directions are proposed to refine N₂O flux control strategies. By consolidating current knowledge and identifying research gaps, this review advances LTS management strategies that improve wastewater treatment efficiency while mitigating the environmental and health impacts of N₂O emissions.

Keywords: Influencing factors; Land treatment systems; N(2)O emission; Nitrogen removal mechanism.