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. 1985 Jan;161(1):432-4.
doi: 10.1128/jb.161.1.432-434.1985.

Isolation and properties of beta-glucosidase from Ruminococcus albus

Isolation and properties of beta-glucosidase from Ruminococcus albus

K Ohmiya et al. J Bacteriol. 1985 Jan.

Abstract

An enzyme active against p-nitrophenyl-beta-D-glucoside was purified from logarithmic-phase cells of Ruminococcus albus cultivated in a medium containing ball-milled cellulose. The purification yielded homogeneous enzyme after an approximately 520-fold increase in specific activity and a 9% yield. The enzyme was identified as a beta-glucosidase because it can hydrolyze cellobiose and cellooligosaccharides to glucose from the nonreducing ends.

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