Fasciola hepatica: interaction of the tegument with poly-L-lysine and enzymes

Abstract

The tegument of Fasciola hepatica was treated with 0.5% pepsin (EC 3.4.4.1), 0.5% alpha-amylase (EC 3.2.1.1), and 0.5% sheep bile solutions both without and following preincubation in poly-L-lysine. Without poly-L-lysine preincubation, pepsin appeared to be breaking down limited areas of the tegumental surface but had no other marked effects on tegumental structure. alpha-Amylase and bile had no major effects on the tegument except for a reduction in matrix density by the latter. Incubation in poly-L-lysine alone resulted in some changes in surface morphology of the tegument and a limited amount of swelling of the basal infolds. When poly-L-lysine was followed by pepsin treatment, blebbing, microvillus-formation, and swelling of the basal infolds was greatly enhanced and led to surface destruction in some areas. alpha-Amylase following poly-L-lysine resulted in complete destruction and loss of the tegument, and left the basal lamina as the external surface, Incubation in bile after poly-L-lysine preincubation resulted in little change in tegumental morphology.