Involvement of the immune system in the pathogenesis of Crohn's disease. Expression of the T9 antigen on peripheral immunocytes correlates with the severity of the disease

Abstract

Peripheral lymphocyte cells from patients suffering from Crohn's disease were analyzed for the expression of the "activation" antigens T9 and HLA-DR on their cell surface. It was found that high numbers of "activated" lymphocytes, the majority of which have proven to be T cells, could be detected in patients with active Crohn's disease, whereas in healthy controls and inactive disease only a small subfraction of lymphocytes was positive for these antigens. This difference was highly significant (p = 0.0001). Within the subpopulation of T9-positive cells the ratio between T4- and T8-positive cells is about 1.8 (compared with 2.0 in the total T-cell subset). All HLA-DR-positive, non-B and non-glass-adherent cells could be detected in the T9-positive cell fraction. The presence of T9 antigens was found to correlate with the grade of severity of the disease as assessed by a Crohn's disease activity index. The presence of high amounts of T cells exhibiting this antigen is not restricted to Crohn's disease but is thought to be of importance as a marker for the involvement of the immune system in other maladies as well. Nevertheless, the determination of T9 antigen is expected to provide objective data reflecting the severity of Crohn's disease.