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. 1985 Mar;38(3):317-9.
doi: 10.1136/jcp.38.3.317.

Enhanced luminescence enzyme immunoassay for factor VIII related antigen

H X Wang, J George, G H Thorpe, R A Stott, L J Kricka, T P Whitehead

Enhanced luminescence enzyme immunoassay for factor VIII related antigen

H X Wang et al. J Clin Pathol. 1985 Mar.

Abstract

A sandwich enzyme immunoassay for plasma factor VIII related antigen has been developed which exploits a para-iodophenol enhanced chemiluminescent reaction to detect the horseradish peroxidase label. The assay entailed 15 min incubations with sample and with conjugate and had a detection limit of 0.12 mU. It showed good within batch precision (coefficient of variation = 2.95-5.8%) and results on a series of 57 specimens agreed with results obtained by immunoelectrophoresis (correlation coefficient = 0.97).

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