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. 2024 Aug 15;14(16):2362.
doi: 10.3390/ani14162362.

A Functional 67-bp Duplication Locating at the Core Promoter Region within the Bovine ADIPOQ Gene Is Associated with Ovarian Traits and mRNA Expression

Yufu Li  1   2 , Tingting Liu  1   3 , Mengyang Zhang  1 , Chuanying Pan  1 , Xu Liu  2 , Haiyu Zhao  4 , Xianyong Lan  1
Affiliations

A Functional 67-bp Duplication Locating at the Core Promoter Region within the Bovine ADIPOQ Gene Is Associated with Ovarian Traits and mRNA Expression

Yufu Li et al. Animals (Basel). .

Abstract

ADIPOQ plays a crucial role in regulating the reproductive system, but there are few reports on the effects of ADIPOQ on ovarian in dairy cows. Previous studies have verified the presence of a 67-bp mutation in the promoter region of the ADIPOQ gene. Hence, we employed ovarian tissues (n = 2111) and blood samples (n = 108) from Chinese Holstein cows as experimental samples to examine the association between ADIPOQ promoter variants and ovarian traits. We extracted DNA from these samples and conducted genetic typing identification on each sample using advanced techniques like PCR and agarose gel electrophoresis. Consequently, the DD, ID, and II genotypes were discovered. and it has been observed that the mutation frequency of this locus is low in the Chinese Holstein cow. Importantly, the correlational analysis unveiled a significant relationship (p < 0.05) between the weight of ovaries in late estrus and the width of ovaries during the estrus interval with the mutation. Result of the RT-PCR revealed that the ID genotype partially diminished the expression of the ADIPOQ gene. The results of this study suggest that the identified variable duplication could serve as a potential genetic marker for enhancing the ovarian traits of Chinese Holstein cows.

Keywords: ADIPOQ; bovine; correlation analysis; ovarian; structural variation.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Research strategies for examining ADIPOQ promoter polymorphisms and ovarian development (created with BioRender.com, accessed on 23 July 2024). (A) Sample collection. (B) Ovarian traits data measurement. (C) DNA extraction. (D) PCR amplification and sequencing. (E) Data analysis. (F) mRNA extraction and RT-PCR.
Figure 2
Figure 2
Identification and analysis of 67-bp duplication in the promoter region of the ADIPOQ gene. (A) The electrophoresis diagrams of the PCR amplification products. DD (471 bp): representing the wild type, contains one copy; ID (471 and 538 bp): defines the heterozygote. (B) Schematic representation of ADIPOQ gene polymorphic loci. (C) The sequence of the 67-bp duplication. II genotype above, DD genotype below. (D) Molecular evolutionary tree of the ADIPOQ gene.
Figure 3
Figure 3
mRNA expression of different genotypes of the cow ADIPOQ gene in ovarian tissue (DD = 3, ID = 3), “*” means: p < 0.05.
See this image and copyright information in PMC

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