Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Aug 8;13(16):2495.
doi: 10.3390/foods13162495.

Anti-Biofilm Effects of Z102-E of Lactiplantibacillus plantarum against Listeria monocytogenes and the Mechanism Revealed by Transcriptomic Analysis

Jinyuan Wei  1 , Xingguo Zhang  1 , Mohamedelfatieh Ismael  1 , Qingping Zhong  1
Affiliations

Anti-Biofilm Effects of Z102-E of Lactiplantibacillus plantarum against Listeria monocytogenes and the Mechanism Revealed by Transcriptomic Analysis

Jinyuan Wei et al. Foods. .

Abstract

Lactic acid bacteria (LAB) are the most common probiotics, and they present excellent inhibitory effects on pathogenic bacteria. This study aimed to explore the anti-biofilm potential of the purified active substance of Lactiplantibacillus plantarum, named Z102-E. The effects of Z102-E on Listeria monocytogenes were investigated in detail, and a transcriptomic analysis was conducted to reveal the anti-biofilm mechanism. The results indicated that the sub-MIC of Z102-E (3.2, 1.6, and 0.8 mg/mL) decreased the bacterial growth and effectively reduced the self-aggregation, surface hydrophobicity, sugar utilization, motility, biofilm formation, AI-2 signal molecule, contents of extracellular polysaccharides, and extracellular protein of L. monocytogenes. Moreover, the inverted fluorescence microscopy observation confirmed the anti-biofilm effect of Z102-E. The transcriptomic analysis indicated that 117 genes were up-regulated and 214 were down-regulated. Z102-E regulated the expressions of genes related to L. monocytogenes quorum sensing, biofilm formation, etc. These findings suggested that Z102-E has great application potential as a natural bacteriostatic agent.

Keywords: Lactiplantibacillus plantarum; Listeria monocytogenes; anti-biofilm; motility; quorum sensing; transcriptomic analysis.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The antibacterial efficacy of Z102-E against L. monocytogenes. (A) The effects of Z102-E on L. monocytogenes growth. (B) The growth curves of L. monocytogenes treated with different concentrations of Z102-E. The significance levels are shown as * p < 0.05 and **** p < 0.0001 compared to the data of control.
Figure 2
Figure 2
The effects of Z102-E on the self-aggregation and surface hydrophobicity. (A) Inhibition effects on self-aggregation. (B) Inhibition effects on surface hydrophobicity. The significance levels are shown as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 compared to the data of control.
Figure 3
Figure 3
The effects of Z102-E on the utilization of different sugars of L. monocytogenes. (A–F) refer to different sugars.
Figure 4
Figure 4
The Z102-E effect on motility of L. monocytogenes. The concentrations of Z102-E from left to right are 3.2, 1.6, 0.8, and 0 mg/mL.
Figure 5
Figure 5
The effects of Z102-E at sub-MICs on the biofilm of L. monocytogenes.
Figure 6
Figure 6
The removal effects of Z102-E on the mature biofilm of L. monocytogenes.
Figure 7
Figure 7
The effects of Z102-E on AI-2 signal molecule, extracellular proteins, and extracellular polysaccharides of L. monocytogenes biofilm.
Figure 8
Figure 8
Inverted fluorescence microscopy observation of cells and EPS in biofilm (400×). (a) live cells; (b) dead cells; (c) EPS; subscripts 1–3 indicate different concentrations of Z102-E (0, 1.6, and 3.2 mg/mL).
Figure 9
Figure 9
Volcano plot of DEGs. Green—significantly down-regulated genes, red—significantly up-regulated genes, gray—genes with no significant changes.
Figure 10
Figure 10
Heatmap of DEGs. Different rows and columns represent different genes and groups of samples, respectively.
Figure 11
Figure 11
The top 20 GO enrichment terms. Enrichment ratio is the ratio of the number of genes annotated to the specific pathway (sample number) to all the genes annotated to the pathways (background number). FDR is a calibrated p-value indicating significance. *** FDR < 0.001, ** FDR < 0.01, * FDR < 0.05.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Davis M.L., Ricke S.C., Donaldson J.R. Establishment of Listeria monocytogenes in the gastrointestinal tract. Microorganisms. 2019;7:75. doi: 10.3390/microorganisms7030075. - DOI - PMC - PubMed
    1. Rodriguez-Auad J.P. Overview of Listeria monocytogenes infection. Rev. Chil. Infectol. 2018;35:649–657. doi: 10.4067/S0716-10182018000600649. - DOI - PubMed
    1. Radoshevich L., Cossart P. Listeria monocytogenes: Towards a complete picture of its physiology and pathogenesis. Nat. Rev. Microbiol. 2018;16:32–46. doi: 10.1038/nrmicro.2017.126. - DOI - PubMed
    1. Osimani A., Clementi F. The occurrence of Listeria monocytogenes in mass catering: An overview in the European Union. Int. J. Hosp. Manag. 2016;57:9–17. doi: 10.1016/j.ijhm.2016.05.005. - DOI
    1. Pennone V., Barron U.G., Hunt K., Cadavez V., McAuliffe O., Butler F. Omnibus modeling of Listeria monocytogenes growth rates at low temperatures. Foods. 2021;10:1099. doi: 10.3390/foods10051099. - DOI - PMC - PubMed

LinkOut - more resources