Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Aug 21;15(8):1102.
doi: 10.3390/genes15081102.

Transcriptome Analysis of Ethylene-Related Genes in Chlorine Dioxide-Treated Fresh-Cut Cauliflower

Weiwei Jin  1   2 , Qiaojun Jiang  1   2 , Haijun Zhao  1   3 , Fengxian Su  1   2 , Yan Li  1   2 , Shaolan Yang  4
Affiliations

Transcriptome Analysis of Ethylene-Related Genes in Chlorine Dioxide-Treated Fresh-Cut Cauliflower

Weiwei Jin et al. Genes (Basel). .

Abstract

Chlorine dioxide (ClO2) is widely used for the quality preservation of postharvest horticultural plants. However, the molecular mechanism of how ClO2 works is not clear. The purpose of this study was to understand ethylene-related molecular signaling in ClO2-treated fresh-cut cauliflower florets. Transcriptome analysis was used to investigate ethylene-related gene regulation. A total of 182.83 Gb clean data were acquired, and the reads of each sample to the unique mapped position of the reference genome could reach more than 85.51%. A sum of 2875, 3500, 4582 and 1906 differential expressed genes (DEGs) were identified at 0 d, 4 d, 8 d and 16 d between the control group and ClO2-treated group, respectively. DEGs were enriched in functions such as 'response to oxygen-containing compounds' and 'phosphorylation', as well as MAPK signaling pathway, plant hormone transduction pathway and so on. Genes, including OXI1, MPK3, WRKY22 and ERF1, which are located at the junction of wounding, pathogen attack, pathogen infection or ethylene signal transduction pathways, were up-regulated in response to stress. ETR and CTR1 (both up-regulated), as well as three down-regulated genes, including BolC5t34953H (a probable NAC), BolC1t05767H (a probable NAC) and BolC2t06548H (a probable ERF13), might work as negative regulators for ethylene signal transduction. In conclusion, ethylene-related genes and pathways are involved in ClO2 treatment, which might enhance stress resistance and have a negative feedback mechanism.

Keywords: RNA-seq; chlorine dioxide; ethylene; fresh-cut cauliflower; stress; transcriptome.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflicts of interest. All authors have read and agreed to the published version of the manuscript.

Figures

Figure 1
Figure 1
PCA analysis of transcriptome data in ClO2 (T) and control (CK) group during storage.
Figure 2
Figure 2
Analysis of DEGs in different comparison periods in ClO2 (T) and control (CK) group of fresh-cut cauliflower florets during storage.
Figure 3
Figure 3
Venn diagram of DEGs in ClO2 (T) and control (CK) group of fresh-cut cauliflower florets during storage.
Figure 4
Figure 4
GO functional enrichment analysis of DEGs at 0 d (A), 4 d (B), 8 d (C) and 16 d (D).
Figure 5
Figure 5
KEGG pathway enrichment analysis of DEGs at 0 d (A), 4 d (B), 8 d (C) and 16 d (D).
Figure 6
Figure 6
Up-regulated DEGs screened from ethylene-related pathways. Different color represents different pathway.
Figure 7
Figure 7
Expression analysis of DEGs involved in ethylene-related pathways.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Avato P., Argentieri M.P. Brassicaceae: A Rich Source of Health Improving Phytochemicals. Phytochem. Rev. 2015;14:1019–1033. doi: 10.1007/s11101-015-9414-4. - DOI
    1. Li Z., Zheng S., Liu Y., Fang Z., Yang L., Zhuang M., Zhang Y., Lv H., Wang Y., Xu D. Characterization of Glucosinolates in 80 Broccoli Genotypes and Different Organs Using UHPLC-Triple-TOF-MS Method. Food Chem. 2021;334:127519. doi: 10.1016/j.foodchem.2020.127519. - DOI - PubMed
    1. Morrison M.E.W., Hobika E.G., Joseph J.M., Stenzel A.E., Mongiovi J.M., Tang L., McCann S.E., Marshall J., Fountzilas C., Moysich K.B. Cruciferous Vegetable Consumption and Pancreatic Cancer: A Case-Control Study. Cancer Epidemiol. 2021;72:101924. doi: 10.1016/j.canep.2021.101924. - DOI - PMC - PubMed
    1. Qinghang W., Zhang C., Zhang J., Xin X., Li T., He C., Zhao S., Liu D. Variation in Glucosinolates and the Formation of Functional Degradation Products in Two Brassica Species during Spontaneous Fermentation. Curr. Res. Food Sci. 2023;6:100493. doi: 10.1016/j.crfs.2023.100493. - DOI - PMC - PubMed
    1. Rasines L., Morera S., Miguel G.S., Artés-Hernández F., Aguayo E. Environmental and Economic Sustainability of Fresh-Cut and Pre-Cooked Vegetables. Sci. Total Environ. 2023;872:162169. doi: 10.1016/j.scitotenv.2023.162169. - DOI - PubMed

MeSH terms

LinkOut - more resources