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. 2024 Aug 8;29(16):3762.
doi: 10.3390/molecules29163762.

Unveiling the Potential of Vitamin D3 Orodispersible Films: A Comprehensive FTIR and UV-Vis Spectroscopic Study

Alfio Torrisi  1 , Mariapompea Cutroneo  2 , Lorenzo Torrisi  2 , Salvatore Lavalle  1 , Alessia Forzina  3 , Francesco Pegreffi  1
Affiliations

Unveiling the Potential of Vitamin D3 Orodispersible Films: A Comprehensive FTIR and UV-Vis Spectroscopic Study

Alfio Torrisi et al. Molecules. .

Abstract

Vitamin D3 is a crucial fat-soluble pro-hormone essential for bolstering bone health and fortifying immune responses within the human body. Orodispersible films (ODFs) serve as a noteworthy formulation strategically designed to enhance the rapid dissolution of vitamin D, thereby facilitating efficient absorption in patients. This innovative approach not only streamlines the assimilation process but also plays a pivotal role in optimizing patient compliance and therapeutic outcomes. The judicious utilization of such advancements underscores a paradigm shift in clinical strategies aimed at harnessing the full potential of vitamin D for improved patient well-being. This study aims to examine the vitamin D3 ODF structure using spectroscopic techniques to analyze interactions with excipients like mannitol. Fourier-transform infrared spectroscopy (FTIR) and ultraviolet-visible (UV-Vis) spectroscopy were utilized to assess molecular composition, intermolecular bonding, and vitamin D3 stability. Understanding these interactions is essential for optimizing ODF formulation, ensuring stability, enhancing bioavailability, and facilitating efficient production. Furthermore, this study involves a translational approach to interpreting chemical properties to develop an administration protocol for ODFs, aiming to maximize absorption and minimize waste. In conclusion, understanding the characterized chemical properties is pivotal for translating them into effective self-administration modalities for Vitamin D films.

Keywords: ATR-FTIR spectroscopy; UV irradiation; UV–Vis–NIR spectroscopy; orodispersible films; vitamin D3.

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Conflict of interest statement

Author Alessia Forzina was employed by the company IBSA Institut Biochimique SA. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Optical microscope photos of the vitamin D3 orodispersible film (ODF) at (a) 10×, (b) 20× (in gray contrast color), and (c) at 50× (real sample color), showing the irregular circular porosity of the surface.
Figure 2
Figure 2
FTIR spectra of the analyzed ODFs (transmittance vs. wavenumber) at different experimental conditions.
Figure 3
Figure 3
(a) Transmittance spectra of the vitamin D3 ODF sample in the wavelength range from 200 to 850 nm before and after its dilution. (b) By increasing the transmittance axis values, it is possible to observe the presence of some characteristic transmittance peaks at high dilution values, which are more evident in (c), where the spectra are represented in the UV region between 200 and 350 nm.
Figure 4
Figure 4
Absorbance spectra vs. wavelength of the ODF sample at different water dilutions (a) in a wide wavelength range (from 200 to 850 nm) and (b) in the UV region (from 200 to 320 nm).
Figure 5
Figure 5
Relative Transmittance vs. wavelength of the ODF sample at different dilutions in water employing (a) a deuterium lamp and (b) a halogen lamp.
Figure 6
Figure 6
Absorption coefficient of vitamin D3 ODF sample in water (100% dilution) vs. wavelength.
Figure 7
Figure 7
Primary (a) and secondary (b) packaging of Vitamin D3 orodispersible films and a film (c).
See this image and copyright information in PMC

References

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