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. 2024 Jul 31;17(8):1008.
doi: 10.3390/ph17081008.

Long Non-Coding H19 in Lymphocytes: Prognostic Value in Acute Ischemic Stroke Patients

Liyuan Zhong  1   2 , Zixian Xie  1   2 , Ziping Han  1   2 , Junfen Fan  1   2 , Rongliang Wang  1   2 , Zhen Tao  1   2 , Qingfeng Ma  2 , Yumin Luo  1   2   3
Affiliations

Long Non-Coding H19 in Lymphocytes: Prognostic Value in Acute Ischemic Stroke Patients

Liyuan Zhong et al. Pharmaceuticals (Basel). .

Abstract

Acute ischemic stroke (AIS) is a cerebrovascular disease that seriously affects the physical and mental health and quality of life of patients. However, there is a lack of reliable prognostic prediction methods. The main objective of this study was to investigate the prognostic value of long non-coding RNA (lncRNA) H19 in lymphocytes of patients with AIS, and to construct a prognostic prediction model for AIS including lncRNA H19 in lymphocytes, which would provide new ideas for the prognostic evaluation of AIS. Poor prognosis was defined when the patient's modified Rankin scale (mRS) score at 3 months after AIS onset was greater than 2. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the level of lncRNA H19 in lymphocytes. Spearman correlation analysis revealed a positive correlation between lncRNA H19 and mRS score at 3 months after AIS onset (r = 0.1977, p = 0.0032), while lncRNA H19 was negatively correlated with white blood cells counts, lymphocytes counts, and neutrophils counts. Logistic regression analysis identified lncRNA H19 as an independent predictor of poor prognosis (OR = 3.062 [1.69-5.548], p < 0.001). Moreover, a nomogram prediction model incorporating lncRNA H19 in lymphocytes demonstrated effective discrimination, calibration, and clinical applicability in predicting AIS outcomes. The findings suggest that lncRNA H19 in lymphocytes could be a valuable prognostic indicator and a potential pharmacological target for AIS patients, and might be a novel pathway for enhanced prognostic evaluation and targeted therapeutic strategies.

Keywords: AIS; lncRNA H19; lymphocytes; prognosis.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Correlation of H19 level in lymphocytes with counts of white blood cells, neutrophils, and lymphocytes. (A) Correlation between H19 level in lymphocytes and white blood cell counts; (B) correlation between H19 level in lymphocytes and neutrophils counts; (C) correlation between H19 level in lymphocytes and lymphocytes counts.
Figure 2
Figure 2
Correlation between H19 level in lymphocytes and prognosis in AIS patients. (A) Correlation between H19 level in lymphocytes and 3-month mRS scores. (B) Expression levels of H19 in lymphocytes of AIS patients with good prognosis (mRS 0–2) and with poor prognosis (mRS 3–6). AIS: acute ischemic stroke. mRS: modified Rankin scale. **** p < 0.0001.
Figure 3
Figure 3
Forest plot for multivariate logistic regression analysis. OR—odds ratio; NLR—neutrophil-to-lymphocyte ratio; NIHSS—National Institutes of Health stroke scale; EMT—endovascular mechanical thrombectomy.
Figure 4
Figure 4
The creation and assessment of the nomogram. (A) The nomogram for predicting 3-month poor prognosis in AIS patients; (B) ROC curves of the nomogram; (C) calibration curves of the nomogram; (D) DCA analysis of the nomogram. EMT—endovascular mechanical thrombectomy; NLR—neutrophil-to-lymphocyte ratio.
See this image and copyright information in PMC

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