Stable Gastric Pentadecapeptide BPC 157 and Intestinal Anastomoses Therapy in Rats-A Review

Abstract

By introducing the healing of many distinctive anastomoses by BPC 157 therapy, this review practically deals with the concept of the resection and reconnection of the hollow parts of the gastrointestinal tract as one of the cornerstones of visceral surgery. In principle, the healing of quite distinctive anastomoses itself speaks for applied BPC 157 therapy, in particular, as a way in which the therapy of anastomoses can be successfully approached and carried out. Some of the anastomoses implicated were esophagogastric, colocolonic, jejunoileal, and ileoileal anastomoses, along with concomitant disturbances, such as esophagitis, sphincter dysfunction, failed intestinal adaptation, colitis, short bowel syndrome, major vessel occlusion, NO-system, and prostaglandins-system dysfunction, which were accordingly counteracted as well, and, finally, findings concerning other anastomoses healing (i.e., nerve and vessel). Moreover, the healing of fistulas, both external and internal, colocutaneous, gastrocutaneous, esophagocutaneous, duodenocutaneous, vesicovaginal, colovesical, and rectovaginal in rats, perceived as anastomoses made between two different tissues which are normally not connected, may also be indicative. This may be a particular reconnection of the parts of the gastrointestinal tract to re-establish adequate integrity depending on the tissue involved, given that both various intestinal anastomoses and various fistulas (intestinal and skin were accordingly healed simultaneously as the fistulas disappeared) were all healed.

Keywords: esophagogastric, colocolonic, jejunoileal, and ileoileal anastomoses; rats; short bowel; stable gastric pentadecapeptide BPC 157; therapy.

Figure 1

Day 7 post operation in rats that received cysteamine 400 mg/kg enema and colon–colon termino-terminal anastomosis (controls small italic letters, red circle; BPC 157 capital italic letters, yellow circle) (a,A). Subileus/ileus in controls (a), and intestine presentation close to normal in BPC 157 rats (10 µg/kg/day in drinking water) (A).

Figure 2

Rats with occlusion of the inferior mesenteric artery and colon–colon anastomosis (A,a,B,b,C,c) in control rats (lower, light color arrows) (small italic letters) and in rats that received BPC 157 in drinking water (upper) (capital italic letters). Superior mesenteric vein (blue arrows) and inferior caval vein (yellow arrows) congested (control, lower) (a) or close to the normal presentation (BPC 157, upper) day 3 post operation (left) (A). Anastomosis dehiscence open (control, lower) (b) or sealed with adhesion (BPC 157, upper) (B) (green arrows) (middle) day 5 post operation. Anastomosis presentation, poor (control, lower) (c) and fully healed (BPC 157, upper) (C) upon sacrifice day 7 post operation (gray arrows) (right).

Figure 3

Rats with ileoileal anastomosis (A,a,B,b,C,c,D,d) in control rats (upper, small italic letters, red indices) and in rats that received BPC 157 (per-orally or intraperitoneally) (lower, capital italic letters, yellow indices). Vessels presenting as empty distal vessels due to anastomosis (control, upper, (a)) or close to the normal presentation, proximal and distal from anastomosis (BPC 157, lower, (A)) day 1 post operation (left). Day 14 post operation. Abundant adhesions in controls (lower, (b)) or less adhesions in BPC 157 rats (lower, (B)) (middle, left). In controls, muscularis mucosa and propria abrupt ending (×2 (c), ×25 (d), control, upper) was observed; in BPC 157 rats, strands of newly formed muscle (×2 (C), ×6.5 (D), BPC 157, lower) (right) were observed.