Advances in Subcellular Accumulation Design for Recombinant Protein Production in Tobacco

Abstract

Plants and their use as bioreactors for the generation of recombinant proteins have become one of the hottest topics in the field of Plant Biotechnology and Plant Synthetic Biology. Plant bioreactors offer superior engineering potential compared to other types, particularly in the realm of subcellular accumulation strategies for increasing production yield and quality. This review explores established and emerging strategies for subcellular accumulation of recombinant proteins in tobacco bioreactors, highlighting recent advancements in the field. Additionally, the review provides reference to the crucial initial step of selecting an optimal subcellular localization for the target protein, a design that substantially impacts production outcomes.

Figure.

Representative proteins and their predominant glycosylation patterns associated with specific subcellular compartments, along with the pros and cons. (A) Representative predominant glycosylation patterns in the specific subcellular compartments. GlcNAc (Gn), N-acetylglucosamine; Man (M), mannose; Xyl (X), xylose; Fuc (F), fucose; Gal, galactose, Le^a, Lewis a. (B) Representative proteins in specific subcellular compartments. The Cryo-EM data of representative VLPs and the PDB structure of representative proteins are flanked with the 4 subcellular compartments. (C) The pros and cons of different subcellular accumulation in tobacco. PTM, posttranslation modification; CT, chloroplast transformation; NT, nuclear transformation.