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Review
. 2024 Aug 31;39(1):229.
doi: 10.1007/s10103-024-04179-9.

Photodynamic therapy on mRNA levels in bacteria

Bruno Ricardo Barreto Pires  1 , Flavia de Paoli  2 , Andre Luiz Mencalha  1 , Adenilson de Souza da Fonseca  3   4   5
Affiliations
Review

Photodynamic therapy on mRNA levels in bacteria

Bruno Ricardo Barreto Pires et al. Lasers Med Sci. .

Abstract

Antimicrobial photodynamic therapy (aPDT) has shown efficacy in inactivating different bacterial species by photosensitizer-induced free radical production. Despite aPDT is considered unable to cause resistant strains, enzymatic pathways for detoxification of reactive oxygen species and transmembrane photosensitizer efflux systems could cause resistance to aPDT. Resistance mechanisms can be evaluated by measurement of mRNA from by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Thus, the aim of this study was to access the mRNA level data obtained by RT-qPCR in bacterial cells submitted to photodynamic therapy. Studies performed on mRNA levels in bacteria after PDT were assessed on MEDLINE/Pubmed. The mRNA levels from genes related to various functions have been successfully evaluated in both Gram-positive and -negative bacteria after aPDT by RT-qPCR. Such an approach has improved the understanding of aPDT-induced effects, and reinforced the effectiveness of aPDT on bacteria, which can cause infections in different human tissues.

Keywords: Bacteria; Gene expression; Photodynamic therapy; RT-qPCR; mRNA.

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References

    1. Lee CN, Hsu R, Chen H, Wong TW (2020) Daylight Photodynamic Therapy: an update. Molecules 25:5195 - PubMed - PMC
    1. Donnelly RF, McCarron PA, Tunney MM (2008) Antifungal photodynamic therapy. Microbiol Res 163:1–12 - PubMed
    1. Yanten N, Vilches S, Palavecino CE (2023) Photodynamic therapy for the treatment of Pseudomonas aeruginosa infections: a scoping review. Photodiagnosis Photodyn Ther 44:103803 - PubMed
    1. Salah A, El-Mahy SK, El-sayed O, Battisha IK (2020) Up-conversion behaviors of nanostructure BaTi0.9Sn0.1O3 activated by Er3+/Yb3 + ions. Opt 209:164571
    1. Atta D, Ismail MM, Battisha IK (2022) 3D laser Raman micro-spectroscopy of Er3 + and Yb3 + co-activated nano-composite phosphosilicate for industrial photonics applications. Opt Laser Technol 149:107761

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