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. 2024 Sep 3;15(1):403.
doi: 10.1007/s12672-024-01282-9.

Diagnostic value and clinical significance of serum miR-4534 combined with transvaginal color Doppler ultrasound in cervical cancer

Xiyan Shao #  1 , Lu Bai #  2 , Jinlan Liang  1 , Ming Li  3
Affiliations

Diagnostic value and clinical significance of serum miR-4534 combined with transvaginal color Doppler ultrasound in cervical cancer

Xiyan Shao et al. Discov Oncol. .

Abstract

Aim: The purpose of the present study was to evaluate the diagnostic performance of serum miR-4534 combined with Transvaginal Color Doppler Ultrasound (TVCDS) in cervical cancer patients.

Methods: Blood samples from 126 patients with cervical cancer and 83 patients with benign uterine lesions were retrospectively analyzed. Quantitative real time polymerase chain reaction (qRT-PCR) was applied to examine the relative abundances of serum miR-4534 in cervical cancer based on a case-control study. Chi-square test was adopted to analyze the association between serum miR-4534 and other clinicopathological factors. The blood flow of cervix was examined using TVCDS, and the blood flow resistance index (RI) of cervix was summarized. Receiver operating characteristic (ROC) curves were plotted to explore the diagnostic capacity of serum miR-4534 combined with blood flow RI. Logistic regression was employed to analyze the risk factors of cervical cancer.

Results: Serum miR-4534 was distinctly increased in the study group compared with the control group (P < 0.05), while blood flow RI was dramatically decreased (P < 0.05). Moreover, increased miR-4534 was closely associated with lymph node metastasis (P = 0.010), FIGO stage (P = 0.007) and HR-HPV (P = 0.025). ROC curves demonstrated that the area under curve (AUC) of serum miR-4534 combined with the blood flow RI was 0.854, with the sensitivity and specificity of 88.9% and 73.5%, respectively, which displayed a better diagnostic capacity than serum miR-4534 and blood flow RI alone. Logistic regression analysis demonstrated that serum miR-4534 (OR = 8.805, 95% CI = 4.124-18.798; P < 0.001) was a risk factor related to the initiation and formation of cervical cancer, as well as blood flow RI (OR = 0.112; 95% CI = 0.054-0.235; P < 0.001).

Conclusion: Serum miR-4534 was highly expressed in cervical cancer, and associated with the development and metastasis of cervical cancer patients. MiR-4534 combined with TVCDS exhibited a considerable biomarker to detect cervical cancer patients.

Keywords: Diagnosis; MiR-4534; RI; ROC; TVCDS.

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Conflict of interest statement

The authors have no relevant financial or non-financial interests to disclose.

Figures

Fig. 1
Fig. 1
Serum miR-4534 expression in cervical cancer patients and the control group. A: Serum miR-4534 expression was distinctly increased in cervical cancer patients compared with controls (P < 0.05); B: The blood flow RI was significantly diminished in cervical cancer patients compared with control group (P < 0.05); C: Serum miR-4534 was negatively correlated with blood flow RI value between two groups via Pearson’s correlation analysis (r = − 0.6073; P < 0.001)
Fig. 2
Fig. 2
ROC curve of serum miR-4534, RI and the combination for diagnosing cervical cancer. A AUC value of serum miR-4534 was 0.811 with the sensitivity of 85.7% and specificity of 72.3% to discriminate the cervical cancer patients from controls. B The AUC value of RI value was 0.787 with the sensitivity of 79.4% and 75.9% to distinguish between cervical cancer patients and controls. C The sensitivity and specificity were 88.9% and 73.5%, respectively, when combining miR-4534 and RI, to detect the cervical cancer
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