[Candida mycoderma growth inhibition with phenol and the autoselection of resistent forms under continuous pH-stat cultivation]

Abstract

The effect of phenol on the growth rate and respiration was studied with the yeast Candida mycoderma cultivated in the pH-static conditions with continuous recording of the principal kinetic parameters of the population. The kinetics of growth inhibition with phenol was studied. Adaptation of the culture in terms of the growth rate and the rate of oxygen uptake was detected within 10--15 hours of cultivation. A new strain of C. mycoderma Phen. R. isolated using the technique of autoselection upon continuous cultivation for a long period of time, at a phenol concentration of 1.5 g/l in the growth medium, had an elevated growth rate (2.2 times higher) in these conditions as compared with the parent culture and required less oxygen.