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. 2024 Aug 3;16(8):e66099.
doi: 10.7759/cureus.66099. eCollection 2024 Aug.

The Incorporation of Chitosan in the Antibacterial Capability and Biocompatibility of a Protein-Repellent Orthodontic Cement

Jianru Yi  1 , Fan He  1 , Zhihe Zhao  1
Affiliations

The Incorporation of Chitosan in the Antibacterial Capability and Biocompatibility of a Protein-Repellent Orthodontic Cement

Jianru Yi et al. Cureus. .

Abstract

Background: This study aimed to develop an orthodontic cement containing chitosan and 2-methacryloyloxyethyl phosphorylcholine (MPC) and to investigate its antibacterial properties and biocompatibility.

Methods: Chitosan and MPC were incorporated into commercial cement. The enamel bonding strength and biocompatibility of the new cement were evaluated. The antibacterial properties were assessed by examining biofilm metabolic activity and colony-forming units (CFU). An evaluation of the protein repellency of the cement was also conducted.

Results: The new cement containing chitosan and MPC had clinically acceptable bonding strength. In comparison to the control, the novel cement demonstrated enhanced protein-repellent properties (p < 0.05), inhibited biofilm metabolic activity (p < 0.05), and reduced CFU counts (p < 0.05) without diminishing cell viability in response to cement extracts (p > 0.05).

Conclusions: The synergistic application of chitosan and MPC endows the cement with potent antibacterial abilities, protein repellency, and favorable biocompatibility.

Keywords: biocompatibility; chitosan; mpc; orthodontic cement; white spot lesions.

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Conflict of interest statement

Human subjects: All authors have confirmed that this study did not involve human participants or tissue. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work.

Figures

Figure 1
Figure 1. Enamel shear bonding strength of orthodontic cements
The same letters indicate no difference between the groups (p > 0.05), while the different letters indicate a significant difference (p < 0.05).
Figure 2
Figure 2. MTT assay of the biofilms on cement disks
The same letters indicate no difference between the groups (p > 0.05), while the different letters indicate a significant difference (p < 0.05).
Figure 3
Figure 3. CFU counts of total S. mutans on cement disks
The same letters indicate no difference between the groups (p > 0.05), while the different letters indicate a significant difference (p < 0.05).
Figure 4
Figure 4. Protein adsorption on cement disks.
The same letters indicate no difference between the groups (p > 0.05), while the different letters indicate a significant difference (p < 0.05).
Figure 5
Figure 5. Cytotoxicity of cement disks
(A) Microscopic observation of human gingival fibroblasts. (B) Cell viability against cement extracts from days 1 and 7. The same letters indicate no difference between the groups (p > 0.05), while the different letters indicate a significant difference (p < 0.05).
See this image and copyright information in PMC

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