Colony stimulating factor 1 receptor (Csf1r) expressing cell ablation in mafia (macrophage-specific Fas-induced apoptosis) mice alters monocyte landscape and atherosclerotic lesion characteristics

Abstract

Macrophage infiltration and accumulation in the atherosclerotic lesion are associated with plaque progression and instability. Depletion of macrophages from the lesion might provide valuable insights into plaque stabilization processes. Therefore, we assessed the effects of systemic and local macrophage depletion on atherogenesis. To deplete monocytes/macrophages we used atherosclerosis-susceptible Apoe^{- /-} mice, bearing a MaFIA (macrophage-Fas-induced-apoptosis) suicide construct under control of the Csf1r (CD115) promotor, where selective apoptosis of Csf1r-expressing cells was induced in a controlled manner, by administration of a drug, AP20187. Systemic induction of apoptosis resulted in a decrease in lesion macrophages and smooth-muscle cells. Plaque size and necrotic core size remained unaffected. Two weeks after the systemic depletion of macrophages, we observed a replenishment of the myeloid compartment. Myelopoiesis was modulated resulting in an expansion of CSF1R^lo myeloid cells in the circulation and a shift from Ly6c^hi monocytes toward Ly6c^int and Ly6c^lo populations in the spleen. Local apoptosis induction led to a decrease in plaque burden and macrophage content with marginal effects on the circulating myeloid cells. Local, but not systemic depletion of Csf1r⁺ myeloid cells resulted in decreased plaque burden. Systemic depletion led to CSF1R^lo-monocyte expansion in blood, possibly explaining the lack of effects on plaque development.

Keywords: Apoptosis; Atherogenesis; Atherosclerosis; Cardiovascular immunology; Cardiovascular inflammation; Colony stimulating factor 1; Csf1r; Inflammation; Innate immunity; MaFIA; Macrophages; Macrophage‐specific Fas‐induced apoptosis; Monocyte infiltration; Monocytes; Myeloid cell depletion.