Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Apr;27(4):619-24.
doi: 10.1128/AAC.27.4.619.

Tobramycin resistance of Pseudomonas aeruginosa cells growing as a biofilm on urinary catheter material

Tobramycin resistance of Pseudomonas aeruginosa cells growing as a biofilm on urinary catheter material

J C Nickel et al. Antimicrob Agents Chemother. 1985 Apr.

Abstract

When disks of urinary catheter material were exposed to the flow of artificial urine containing cells of Pseudomonas aeruginosa, a thick adherent biofilm, composed of these bacteria and of their exopolysaccharide products, developed on the latex surface within 8 h. After this colonization, sterile artificial urine containing 1,000 micrograms of tobramycin per ml was flowed past this established biofilm, and a significant proportion of the bacterial cells within the biofilm were found to be still viable after 12 h of exposure to this very high concentration of aminoglycoside antibiotic. Planktonic (floating) cells taken from the test system just before the exposure of the biofilm to the antibiotic were completely killed by 50 micrograms of tobramycin per ml. The MIC of tobramycin for cells taken from the seeding cultures before colonization of the catheter material, and for surviving cells recovered directly from the tobramycin-treated biofilm, was found to be 0.4 micrograms/ml when dispersed cells were assayed by standard methods. These data indicate that growth within thick adherent biofilms confers a measure of tobramycin resistance on cells of P. aeruginosa.

PubMed Disclaimer

References

    1. J Ultrastruct Res. 1969 Jan;26(1):31-43 - PubMed
    1. Stanford Med Bull. 1955 May;13(2):290-1 - PubMed
    1. J Infect Dis. 1976 Jan;133(1):14-21 - PubMed
    1. J Bacteriol. 1979 May;138(2):609-17 - PubMed
    1. Annu Rev Microbiol. 1979;33:459-79 - PubMed

Publication types

MeSH terms

LinkOut - more resources